Abstract
DNA gyrase is a type II DNA topoisomerase found in bacteria. Since its discovery in 1976 (Geliert et al. 1976), it has been the focus of a great deal of research, from both mechanistic and medical viewpoints, and it is the purpose of this chapter to address the first of these two areas.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ali JA, Jackson AP, Howells AJ, Maxwell A (1993) The 43 kDa N-terminal fragment of the DNA gyrase B protein hydrolyses ATP and binds coumarin drugs. Biochemistry 32:2717–2724
Celia H, Hoermann L, Schultz P, Lebeau L, Mallouh V, Wigley DB, Wang JC, Mioskowski C, Oudet P (1994) Three-dimensional model of E. coli gyrase B subunit crystallised in two dimensions on novobiocin-linked phospholipid films. J Mol Biol 236:618–628
Geliert M, Mizuuchi K, O’Dea MH, Nash HA (1976) DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc Natl Acad Sci USA 73:3872–3876
Jackson AP, Maxwell A (1993) Identifying the catalytic residue of the ATPase reaction of DNA gyrase. Proc Natl Acad Sci USA 90:11232–11236
Kirchhausen T, Wang JC, Harrison SC (1985) DNA gyrase and its complexes with DNA: direct observation by electron microscopy. Cell 41:933–943
Kreuger S, Zaccai G, Wlodawer A, Langowski J, O’Dea M, Maxwell A, Geliert M (1990) Neutron and light-scattering studies of DNA gyrase and its complex with DNA. J Mol Biol 211:211–220
Lewis RJ, Singh OMP, Smith CV, Maxwell A, Skarzynski T, Wonnacott AJ, Wigley DB (1994) Crystallisation of inhibitor complexes of an N-terminal 24-kDa fragment of the DNA gyrase B protein. J Mol Biol 241:128–130
Maxwell A (1993) The interaction between coumarin drugs and DNA gyrase. Mol Microbiol 9:681–686
Rau DC, Geliert M, Thoma F, Maxwell A (1987) Structure of the DNA gyrase-DNA complex as revealed by transient electric dischroism. J Mol Biol 193:555–569
Reece RJ, Maxwell A (1989) Tryptic fragments of the E. coli DNA gyrase A protein. J Biol Chem 264:19648–19653
Roca J, Wang JC (1992) The capture of a DNA double helix by an ATP-dependent protein clamp: a key step in DNA transport by type II topoisomerases. Cell 71:833–840
Roca J, Wang JC (1994) DNA transport by a type II topoisomerase: evidence in favour of a two-gate mechanism. Cell 77:609–616
Wigley DB, Davies GJ, Dodson EJ, Maxwell A, Dodson GG (1991) The crystal structure of an N-terminal fragment of the DNA gyrase B protein. Nature 351:624–629
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Wigley, D.B. (1995). Structure and Mechanism of DNA Gyrase. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79488-9_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-79488-9_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79490-2
Online ISBN: 978-3-642-79488-9
eBook Packages: Springer Book Archive