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The Cytoskeleton pp 117–126Cite as

Actin and Actin-Binding Proteins in the Motility of Dictyostelium

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Abstract

Dictyostelium discoideum cells are highly motile throughout all stages of development and directed cell motility is essential for morphogenesis to occur (Williams and Jermyn 1991). Cells move by pseudopod extension, and formation and retraction of pseudopods in response to a chemotactic stimulus is critical for directed migration. Folate acts as chemotactic agent during growth, cAMP is the active compound during development. The understanding of chemotactic signaling has advanced considerably (Devreotes 1989), but its linkage to intracellular changes that lead to cell motility is less clear (reviewed in Schleicher and Noegel 1992). Early observations indicated that chemoattractant-induced pseudopod formation correlates temporally and spatially with the polymerization of actin, and peaks of F-actin occur by 15–60 s after stimulation (McRobbie and Newell 1983; Hall et al. 1988). Five different steps in actin changes can be distinguished (Fig. 1), and these steps were found to coincide with retraction of the existing pseudopod and generation of new pseudopods (Dhamaward- hane et al. 1989). Parallel processes are an influx of Ca2+ and an efflux of protons leading to an alkalinization of the cytoplasm. The phosphoinositides are also affected with an increase of IP3 as fast as 6 s after stimulation at the expense of PIP and PIP2 (Europe-Finner et al. 1991).

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Author information

Authors and Affiliations

  1. Institut für Zellbiologie, Max-Planck-Institut für Biochemie, 82152, Martinsried, Germany

    A. A. Noegel, B. Köppel, U. Gottwald, W. Witke & R. Albrecht

  2. Ludwig-Maximilians-Universität, 80336, Munich, Germany

    M. Schleicher

Authors
  1. A. A. Noegel
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  2. B. Köppel
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  3. U. Gottwald
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  4. W. Witke
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  5. R. Albrecht
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  6. M. Schleicher
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Editor information

Editors and Affiliations

  1. Zoologisches Institut Abteilung Zellbiologie, Technische Universität Braunschweig, Spielmannstr.7, D-38092, Braunschweig, Germany

    B. M. Jockusch

  2. AG für strukturelle Molekularbiologie, Max-Planck-Gesellschaft, Notkestr. 85, D-22607, Hamburg, Germany

    E. Mandelkow

  3. Max-Plank-Institut für Biophysikalische Chemie, Am Faßberg, D-37018, Göttingen, Germany

    K. Weber

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© 1995 Springer-Verlag Berlin Heidelberg

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Noegel, A.A., Köppel, B., Gottwald, U., Witke, W., Albrecht, R., Schleicher, M. (1995). Actin and Actin-Binding Proteins in the Motility of Dictyostelium . In: Jockusch, B.M., Mandelkow, E., Weber, K. (eds) The Cytoskeleton. Colloquium der Gesellschaft für Biologische Chemie 14.–16. April 1994 in Mosbach/Baden, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79482-7_13

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  • DOI: https://doi.org/10.1007/978-3-642-79482-7_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79484-1

  • Online ISBN: 978-3-642-79482-7

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