Biomarkers in Ecotoxicology: an Overview

  • J. López-Barea
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 17)


Human activities induce stress on ecological systemsby importing pollutants, modifying habitats, introducing exotic species or removing the native ones and by changing climate. These activities not only affect the survival or the performance of individual organisms, but also the structure and function of natural ecosystems and the diversity of life at several levels of organisation, including the number of species, the genetic composition, and the variety of ecosystems and landscapes (Steinberg et al. 1994).


Stress Protein Lewis Publisher Contaminant Exposure Aquatic Toxicology Adenylate Energy Charge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Adams SM, Shepard KL, Greely MS Jr, Jimenez BD, Ryon MG, Shugart LR, McCarthy JF (1989) Mar Environ Res 28:459–464CrossRefGoogle Scholar
  2. Andersson T, Förlin L(1992) Regulation of the cytochrome P450 enzyme system in fish. Aquat Toxicol 24:1–19CrossRefGoogle Scholar
  3. Bennet WP, Colby TV, Travis WD, Borkowski A, Jones RT, Lane DP, Metealf RA, Samet JM, Takeshima Y, Gu JR, Vähäkangas KH, Soini Y, Pääkkö P, Welsh JA, Trump BF, Harris CC (1993) p53 protein accumulates frequently in early bronchial neoplasia. Cancer Res 53:4817–4822Google Scholar
  4. Boon JP, Everaarts JM, Hillebrand MTJ, Eggens MJ, Pijnenburg J, Goksoyr A (1992) Changes in levels of hepatic biotransformation enzymes and hemoglobin levels in female plaice (Pleuronectes platessa) after oral administration of a technical poly- chlorinated biphenyl mixture (Clophen A40). Sci Total Environ 114:113–133PubMedCrossRefGoogle Scholar
  5. Britvic S, Kurelec B (1986) Selective activation of carcinogenic aromatic amines to bacterial mutagens in the marine mussel Mytilus galloprovincialis. Comp Biochem Physiol 85C: 111–114Google Scholar
  6. Brandt-Rauf PW (1991) Oncogene proteins as biomarkers in the molecular epidemiology of occupational carcinogenesis. The example of the ras oncogene-encoded p21 protein. Int Arch Occup Environ Health 63:1–8PubMedCrossRefGoogle Scholar
  7. Brock V (1993) Effects of mercury on physiological condition and content of the biomarker ALA in the oyster Ostrea edulis. Mar Ecol Progr Ser 96:169–175CrossRefGoogle Scholar
  8. Castellani S, Mattei N, Renzoni A, Wdker CH, Savva D (1993) Preliminary studies on the use of PCR and DNA fingerprints to detect the genotoxic effects of environmental chemicals. Proceedings of the First SETAC World Congress, Lisbon, pp. 247–247Google Scholar
  9. Chan KM, Davidson WS, Hew CL, Fletcher GL (1989) Molecular cloning of metalloth- ionein cDNA and analysis of metallothionein gene expression in winter flounder tissues. Can J Zool 67:2520–2529CrossRefGoogle Scholar
  10. Chipman JK, Marsh JW (1991) Bio-techniques for the detection of genetic toxicity in the aquatic environment. J Biotechnol 17:199–208PubMedCrossRefGoogle Scholar
  11. Courtenay S, Grunwald C, Kreamer G-L, Alexander R, Wirgin I (1993) Induction and clearance of cytochrome P4501A mRNAin Atlantic tomcod caged in bleached kraft mill effluent in the Miramichi River. Aquat Toxicol 27:225–244CrossRefGoogle Scholar
  12. Darnell J, Lodish H, Baltimore D (1990) Molecular Cell Biology, Scientific American Books, IncGoogle Scholar
  13. De Flora S, Vigano L, D’Agostini F, Camoirano A, Bagnasco M, Bennicelli C, Melodia F, Arillo A (1993) Multiple genotoxicity biomarkers in fish exposed in situ to polluted river water. Mutation Res 319:167–177PubMedCrossRefGoogle Scholar
  14. Degan P, Shigenaka MK, Park E-M, Alperin P, Ames BN (1991) Immunoaffinity isolation of urinary 8-hydroxy-2’-deoxyguanosine and 8-hydroxyguanine and quantification of 8-hydroxy-2’-deoxyguanosine in DNA by polyclonal antibodies. Carcinogenesis 12:865–871PubMedCrossRefGoogle Scholar
  15. Depledge MH (1989) The rational basis for detection of early effects of marine pollutants using physiological indicators. Ambio 18:301–302Google Scholar
  16. Dieter MP (1979) Blood 5-aminolevulinic acid dehydratase (ALA-D)to monitor lead contamination incanvasback ducks (Aythya vali sine ria). In: Animals as Monitors of Environmental Pollutants. National Academy of Sciences, Washington DC, p. 177Google Scholar
  17. Di Giulio RT, Wshburn PC, Winning R J, Winston GW, Jewell CS (1989) Biochemical responses in aquatic animals: a review of determinants of oxidative stress. Environ Toxicol Chem 8:1103–1123CrossRefGoogle Scholar
  18. Dunn BP (1991) Carcinogen adducts as an indicator for the public health risks of consuming carcinogen-exposed fish and shellfish. Environ Health Perspect 90:111–116PubMedCrossRefGoogle Scholar
  19. Ellis RJ (1990) The molecular cheperon concept. Semin Cell Biol 1:1–13PubMedGoogle Scholar
  20. Endicott JA, Ling V (1989) The biochemistry of P-glycoprotein-mediated multidrug resistance. Annu Rev Biochem 58:137–165Google Scholar
  21. Engel EW, Roesijadi G (1987) Metallothioneins: a monitoring tool. In: Pollution and Physiology of Estuarine Organisms, Vcrnberg WB, Thurberg FP, Calabrese A, and Vrnberg WB (eds), University of South Carolina Press, Columbia, pp. 421–438Google Scholar
  22. Fossi C, Leonzio C, Focardi S (1986) Increase in organochlorines and MFOactivity in water birds wintering in an Italian lagoon. Bull Environ Contam Toxicol 37:538–543PubMedCrossRefGoogle Scholar
  23. Fossi C, Leonzio C (eds) (1994) Nondestructive Biomarkers in Vertebrates, Lewis Publishers, Boca RatónGoogle Scholar
  24. Goksøyr A, Forlin L (1992) The cytochrome P-450 system in fish aquatic toxicology and environmental monitoring. Aquatic Toxicol. 22:287–312CrossRefGoogle Scholar
  25. Granick S, Sassa S, Granick JL, Levere RD, Kappas A (1972) Assays for porphyrins, 5- aminolevulinic acid dehydratase, and porphyrinogen synthase in microliter samples of whole blood: Application to metabolic defects involving the heme pathway. Proc Natl Acad Sci USA 69:2381–2385PubMedCrossRefGoogle Scholar
  26. Grant SG, Bigbee WL (1993) In vivo somatic mutation and segregation at the human gly- cophorin A (GPA)locus: phenotypic variation encompassing both gene-specific and chromosomal mechanisms. Mutation Res 288:163–172PubMedCrossRefGoogle Scholar
  27. Grue CE, Hart ADM, Mineau P (1992) Biological consequences of depressed brain cholinesterase activity in wild life. In: Cholinesterase-inhibiting Insecticides: Impact on Wildlife and the Environment, Mineau P(ed), Elsevier, Amsterdam, pp. 151- 212Google Scholar
  28. Gupta RC, Randerath K (1988) Analysis of DNAadducts by 32Plabelling and thin-layer chromatography. In: DNA Repair Vol 3, Friedberg E, Hanawalt PH (eds), Marcel Dekker, New York, pp. 399–418Google Scholar
  29. Hartl FU, Martin J, Neupert W (1992) Protein folding in the cell: the role of molecular chaperones Hsp70 and Hsp60. Annu Rev Biophys Biomol Struct 21:293–325PubMedCrossRefGoogle Scholar
  30. Haux C, Förlin L (1988) Biochemical methods for detecting effects of contaminants in fish. Ambio 17:376–380Google Scholar
  31. Heilman LJ, Sheen YY, Bigelow SW, Nebert DW (1988) Trout P450IA1: cDNAand deduced protein sequence, expression in liver, and evolutionary significance. DNA 7:379–387CrossRefGoogle Scholar
  32. Hinton DE, Laurén DJ (1990) Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. In: Biomarkers of Environmental Contamination, McCarthy JF and Shugart LR(eds), Lewis Publishers, Boca Ratón, pp. 17–57Google Scholar
  33. Huggett RA, Kimerle RA, Mehrle PMJr, Bergman HL (eds) (1992) Biomarkers: Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Lewis Publishers, Boca RatonGoogle Scholar
  34. Hunsaker CT (1993) New concepts in environmental monitoring: the question of indicators. Sei Total Env suppl 1993: 77–95CrossRefGoogle Scholar
  35. Jaylet A, Depario P, Ferrier Y, Grinfeld S, Siboulet R (1986) Anew micronucleus test using peripheral blood erythrocytes of the newtPleurodeles walte to detect mutagens in fresh water, Mutation Res 164:245–257PubMedGoogle Scholar
  36. Jiménez BD, Oikari A, Adams SM, Hinton DE, McCarthy JF (1990) Hepatic enzymes as biomarkers: interpreting the effects of environmental, physiological and toxicolog- ical variables. In: Biomarkers of Environmental Contamination. McCarthy JF, Shugart LR (eds), Lewis Publishers, Boca Ratón, pp. 123–142Google Scholar
  37. Jones NJ, Parry JM (1992) The detection of DNAadducts, DNA base changes and chromosome damage for the assessment of exposure to genotoxic pollutants. Aquat Toxicol 22:323–344CrossRefGoogle Scholar
  38. Kagi JHR, Kojima Y (1987) Chemistry and biochemistry of metallothioneins. In: Metallothionein II. Kagi JHR, Kojima Y (eds), Birkhauser-Verlag, Basel, pp. 25–52Google Scholar
  39. Kille P, Kay J, Leaver M, George S (1992) Induction of piscine metallothionein as a primary response to heavy metal pollutants: applicability of new sensitive molecular probes. Aquat Toxicol 22:279–286CrossRefGoogle Scholar
  40. Kurelec B, Matijasevic Z, Rijavec M, Alacevic M, Britvic S, Müller WG, Zahn RK (1979) Induction of benzo(a)pyrene monooxygenase in fish and the Salmonelle test as a tool for detecting mutagenic/carcinogenic xenobiotics in the aquatic environment. Environ Contam Toxicol 21:799–807CrossRefGoogle Scholar
  41. Kurelec B, Garg A, Krca S, Chacko M, Gupta RC (1989) Natural environment surpasses polluted environments in inducing DNA damage in fish. Carcinogenesis 10:1337–1339PubMedCrossRefGoogle Scholar
  42. Kurelec B, Pivcevic B (1991) Evidence for a multixenobiotic resistance mechanism in the mussel Mytilus galloprovincialis. Aquat Toxicol 19:291–302CrossRefGoogle Scholar
  43. Kurelec B (1992) The multixenobiotic resistance mechanism in aquatic organisms. Crit RevToxicol 22:23–43Google Scholar
  44. Lemaire P, Mathieu A, Giudicelli J, LaFaurie M (1992) Effect of diet on the responses of hepatic biotransformation enzymes to benzo(a)pyrene in the European sea bass (Dicentrarchus labrax). Comp Biochem Physiol 102:413–420Google Scholar
  45. Levine AJ, Momand J, Finlay CA (1991) The p53 tumour suppressor gene. Nature 351:453–456PubMedCrossRefGoogle Scholar
  46. Livingstone DR (1993) Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. J Chem Tech Biotechnol 57:195–211Google Scholar
  47. Lowe WR, Kendall RJ(1990) Sentinel species and sentinel bioassay. In: Biomarkers of Environmental Contamination. McCarthy JF, Shugart LR(eds), Lewis Publishers, Boca Raton, pp. 309–331Google Scholar
  48. Lubet RA, Nims RV, Beebe LE, Fox SD, Issaq HJ, McBee K (1992) Induction of hepatic CIP1A activity as a biomarker for environmental exposure to Aroclor 1254 in feral rodents. Arch Environ Contam Toxicol 22:339–344PubMedCrossRefGoogle Scholar
  49. Maccubbin AE (1994) DNAadduct analysis in fish: laboratory and field studies. In: Aquatic Toxicology. Molecular, Biochemical and Cellular Perspectives. Malins DC, Ostrander GK (eds), Lewis Publishers, Boca Raton, pp.261–294Google Scholar
  50. Majone F, Brunetti R, Gol I, Levis AA (1987) Persistence of micronuclei in the marine mussel, Mytilus galloprovincialis after treatment with mitomycin C. Mutation Res 191:157–161PubMedCrossRefGoogle Scholar
  51. Malins DC (1993) Identification of hydroxyl radical-induced lesions in DNAbase structure: biomarkers with a putative link to cancer development. JToxicol Environ Health 40:247–261CrossRefGoogle Scholar
  52. Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113:173–215PubMedGoogle Scholar
  53. Marsh JW, Chipman JK, Livingstone DR (1993) Formation of DNAadducts following laboratory exposure of the mussel, Mytilus edulis, to xenobiotics. Sci Total Environ suppl 1993:567–572CrossRefGoogle Scholar
  54. Martinez-Lara E, Pascual P, Toribio F, Lopez-Barea J, Barcena JA (1992) Arapid method for the quantification of glutathione transferase isoenzymes in crude extracts. J Chromatogr 609:141–146PubMedCrossRefGoogle Scholar
  55. McCarthy JF, Shugart LR (eds) (1990) Biomarkers of Environmental Contamination. Lewis Publishers, Boca RatonGoogle Scholar
  56. Moal J, le Coz JR, Samain JF, Daniel JY (1991) Adenylate energy charge: a possible trophic index for management of oyster intensive aquaculture. Comp Biochem Physiol 100C:201–205Google Scholar
  57. Mohn GR, de Raat WK (1993) Ecological significance of mutagens. Sci Total Environ suppl 1993:1771–1778Google Scholar
  58. Moore MN, Livingstone DR, Widdows J, Lowe DM, Pipe RK (1987) Molecular, cellular and physiological effects of oil-derived hydrocarbons on molluscs and their use in impact assessment. Phil Trans R Soc London B 316:603–623CrossRefGoogle Scholar
  59. Moore MN (1990) Lysosomal cytochemistry in marine environmental monitoring. Histochem J 22:187–191PubMedCrossRefGoogle Scholar
  60. Moore MN, Simpson MG (1992) Molecular and cellular pathology in environmental impact assessment. Aquat Toxicol 22:313–322CrossRefGoogle Scholar
  61. Moore MN (1993) Biomarkers of contaminant exposure and effect: a way forward in marine environmental toxicology. Sci Total Environ suppl 1993:1335–1343Google Scholar
  62. Moore MN, Chipman JK, denBesten PJ, Kurelec B, Bergman A (1993) Sci Total Env suppl 1993:1767–1770Google Scholar
  63. Murphy TA, Kaputska L (1990) Capabilities and limitations of approaches to in situ ecological evaluation. In: In situ Evaluation of Biological Hazards of Environment- al Pollutants. Sandhu SS, Lower WR, deSerres FJ, Suk WA, Tice RR(eds). Plenum Press, New YorkGoogle Scholar
  64. Myers MS, Landahl JT, Krahn M, McCain BB (1991) Relationships between hepatic lesions and exposure to toxic chemicals in marine fish from the US west coast. Environ Health Perspect 90:7–15PubMedCrossRefGoogle Scholar
  65. Nebert EW, Petersen DD, Fornace AJ (1990) Cellular responses to oxidative stress: the [Ah] gene battery as a paradigm. Environ Health Perspect 88:13–25PubMedCrossRefGoogle Scholar
  66. Nebert EW, Nelson DR, Coon MJ, Estabrook RV, Feyereisen R, Fujii-Kuriyama Y, González FJ, Guengerich FP, Gunsalus IC, Johnson EF, Loper JC, Sato R, Witerman MR, Wixman DJ (1991) The P450- superfamily: update on new sequences, gene mapping, and recommended nomenclature. DNA Cell Biol 10:1–14PubMedCrossRefGoogle Scholar
  67. Norey CG, Lees WE Darke BM, Stark JM, Baker TS, Cryer A, Kay J (1990) Immunological distinction between piscine and mammalian metallothioneins. Comp Biochem Physiol 95B:597–601Google Scholar
  68. Okey AB (1990) Enzyme induction in the cytochrome P-450 system. Pharmacol Therapeut 45:241–298CrossRefGoogle Scholar
  69. Oren O (1992) p53: the ultimate tumor suppressor gene? FASEB J 6:3169–3176Google Scholar
  70. Park J-W, Cundy KC, Ames BN (1989) Detection of DNAadducts by high performance liquid chromatography with electrochemical detection. Carcinogenesis 10:827–832PubMedGoogle Scholar
  71. Parry JM, Shamsher M, Skibinski DOF (1990) Restriction site mutation analysis, a proposed methodology for the detection and study of DNA base changes following mutagen exposure. Mutagenesis 5:209–212PubMedCrossRefGoogle Scholar
  72. Pavicic B, Raspor D, Martincic D (1993) Quantitative determination of metallothionein- like proteins in mussels. Methodological approach and field evaluation. Marine Biol 115:435–444CrossRefGoogle Scholar
  73. Peakall DB (1994) Biomarkers. The way forward in environmental assessment. Toxicol and Ecotoxicol News 1:55–60Google Scholar
  74. Peakall DB, Shugart LR (1993) Biomarkers. Research and Application in the Assessment of Environmental Health. Springer Verlag, BerlinGoogle Scholar
  75. Pedrajas JR, Peinado J, López-Barea J (1993) Purification of isoforms of Cu, Zn- superoxide dismutase from fish liver: appearance of new isoforms as consequence of pollution. Free Rad Res Commun 19:29–41CrossRefGoogle Scholar
  76. Perera F, Brenner D, Jeffrey A, Mayer J, Tang D, Wirburton D, Young T-I, Wizneh U Latriano L, MotykiewiczG, Grzybowska E, Chorazy M, HemminkiK, Santella R(1992) DNAadducts and related biomarkers in populations exposed to environmental carcinogens. Environ Health Perspect 98:133–137Google Scholar
  77. Poirier MC (1984) The use of carcinogen -DNA adduct antisera for quantitation and localisation of genomic damage in animal models and human population. Environ Mutagen 6:879–887PubMedCrossRefGoogle Scholar
  78. Rattner BA, Hoffman DJ, Marn CM (1989) Use of mixed-function oxygenases to monitor contaminant exposure in wildlife. Environ Toxicol Chem 8:1093–1102CrossRefGoogle Scholar
  79. Rodriguez-Ariza A, Abril N, Navas JI, Dorado G, López-Barea, Pueyo C (1992) Metal, mutagenicity and Biochemical studies on bivalve molluscs from Spanish coasts. Environ Molec Mutagen 19:112–124CrossRefGoogle Scholar
  80. Rodriguez-Ariza A, Martínez-Lara E, Pascual P, Pedrajas JR, Abril N, Dor ado G, Toribio F, Bárcena JA, Peinado J, Pueyo C, López-Barea J (1993a) Biochemical and genetic indices of marine pollution in Spanish littoral. Sci Total Environ suppl 1993:109–116CrossRefGoogle Scholar
  81. Rodriguez-Ariza A, Peinado J, Pueyo C, López-Barea J (1993b) Biochemical indicators of oxidative stress in fish from polluted littoral areas. Can J. Fish Aquat Sci 50:2568–2573CrossRefGoogle Scholar
  82. Rodriguez-Ariza A, Dorado G, Navas JI, Pueyo C, López-Barea J (1994a) Promutagen activation by fish liver as biomarker of littoral pollution. Environ Molec Mutagen, in pressGoogle Scholar
  83. Rodriguez-Ariza A, Toribio F, López-Barea J (1994b) Rapid determination of glutathione status in fish using HPLC and electrochemical detection. J Chromatogr Biomed Appl 656:311–318CrossRefGoogle Scholar
  84. Roesijadi G, Robinson WE (1994) Metal regulation in aquatic animals: mechanisms of uptake, accumulation, and release. In: Aquatic Toxicology. Molecular, Biochemical and Cellular Perspectives. Malins DC, Ostrander GK (eds), Lewis Publishers, Boca Ratón, pp. 387–420Google Scholar
  85. Rothman JE (1989) Polypeptide chain binding proteins: catalysts of protein folding and related processes in cell. Cell 59:591–602PubMedCrossRefGoogle Scholar
  86. Sanders BM (1993) Stress proteins in aquatic organisms: an environmental perspective. Crit Rev Toxicol 23:49–75PubMedCrossRefGoogle Scholar
  87. Sanders BM, Martin LS (1993) Stress proteins as biomarkers of contaminant exposure in archived environmental samples. Sci Total Environ 139/140: 459–470PubMedGoogle Scholar
  88. Santella RM (1991) DNAadducts in humans as biomarkers to environmental and occupational carcinogens. Environ Carcino Ecotox Revs C9:57–81Google Scholar
  89. Schmitt CJ, Wildhaber ML, Hunn JB, Nash T, Tieger MN, Steadman BL (1993) Biomonitoring of lead-contaminated Missouri streams with an assay for erythrocyte 8-aminolevulinic acid dehydratase activity in fish blood. Arch Environ Contain Toxicol 25:464–475CrossRefGoogle Scholar
  90. Sheehan D (1994) Environmental Biochemistry. The Biochemist June/July:34–37Google Scholar
  91. Shin DM, Kim J, Ro J, Hittelman J, Roth JA Hong WK, Hittelman WN (1994) Activation of p53 gene expression in premalignant lesions during head and neck tumorigenesis. Cancer Res 54:321–326PubMedGoogle Scholar
  92. Stegeman JJ (1989) Cytochrome P-450 forms in fish: catalytic, immunological and sequence similarities. Xenobiotica 19:1093–1110PubMedCrossRefGoogle Scholar
  93. Stegeman JJ, Lech JJ (1991) Cytochrome P-450 monooxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure. Environ Health Perspect 90:101–109PubMedCrossRefGoogle Scholar
  94. Stegeman JJ, Brouwer M, DiGiulio RT, Forlin L, Fowler B, Sanders BM, van Veld PA (1992) Molecular responses to environmental contamination: enzyme and protein synthesis as indicators of chemical exposure and effect. In: Biomarkers: Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Huggett RA, Kimerle RA, Mehrle PMJr, Bergman HL (eds), Lewis Publishers, Boca Ratón, pp 235–335Google Scholar
  95. Steinberg CEW, Geyer HJ, Kettrup AAF (1994) Evaluation of xenobiotic effects by ecological techniques. Chemosphere 28:357–374CrossRefGoogle Scholar
  96. Taguchi M, Witanabe S, Sato Y, Kameya T, Munakata N, Ishihara K, Nakane PK, Hisatome H, Ikeda S (1993) Immunohistochemical examination of tumor-suppressor genep53 product and pyrimidine dimer in solar keratosis. J Cancer Res Clin Oncol 119:260–262PubMedCrossRefGoogle Scholar
  97. Tannenbaum SR (1990) Hemoglobin-carcinogen adducts as molecular biomarkers in epidemiology. In: Princess Takamatsu Symposia Vol 21, Japan Scientific Society Press, Tokyo, pp. 351–360Google Scholar
  98. Timbrell JA, Draper R, Witerfield CJ (1994) Biomarkers in Ecotoxicology. Newuses for some old molecules? Toxicol and Ecotoxicol News 1:4–14Google Scholar
  99. Uchida K, Kawakishi S (1993) 2-Oxo-histidine as a novel biological marker for oxidatively modified proteins. FEBS Lett 332:208–210PubMedCrossRefGoogle Scholar
  100. van Beneden RJ, Ostrander GK (1994) Expression of oncogenes and tumor suppressor genes in teleost fishes. In: Aquatic Toxicology. Molecular, Biochemical and Cellular Perspectives. Malins DC, Ostrander GK (eds), Lewis Publishers, BocaRaton, pp. 295–325Google Scholar
  101. Vkranasi U, Stein JE, Nishimoto M, Reichert WL, Collier TK (1987) Chemical carcinogenesis in feral fish: uptake, activation, and detoxication of organic xenobiotics. Environ Health Perspect 71:155–170CrossRefGoogle Scholar
  102. Wilker CH (1992) Biochemical responses as indicators of toxic effects of chemicals in ecosystems. Toxicol Lett 64/65:527–533CrossRefGoogle Scholar
  103. Wing L-D, Hong J-Y, Qiu S-L, Gao H, Yang CS (1993) Accumulation of p53 protein in human esophageal precancerous lesions: a possible early biomarker for carcinogen-esis. Cancer Res 53:1783–1787Google Scholar
  104. Widdows J, Donkin P (1991) Role of physiological energetics in ecotoxicology. Comp Biochem Physiol 100C:69–75Google Scholar
  105. Wild CP, Jiang YZ, Allen SJ, Jansen LA, Hall AJ, Montesano R (1990) Aflatoxin-albumin adducts in human sera from different regions of the world. Carcinogenesis 11:2271–2274PubMedCrossRefGoogle Scholar
  106. Wild CP, Hudson G, Sabbioni G, Wogan GN, Whittle H, Montesano R, Groopman JD (1992) Correlation of dietary intake of aflatoxins with the level of albumin bound aflatoxin in peripheral blood in The Gambia, West Africa. Cancer Epidemiol, Biomarkers Prev 1:229–234Google Scholar
  107. Zafarullah M, Olsson P-E, Gedamu L (1989) Endogenous and heavy metal ion induced metallothionein gene expression in salmonid tissues and cell lines. Gene 83:85–93PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • J. López-Barea
    • 1
  1. 1.Departamento de Bioquímica yBiología Molecular (Facultad de Veterinaria) eInstituto de Biología Básica y AplicadaUniversidad de CórdobaCórdobaSpain

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