Abstract
Gene technology had an extensive impact on the understanding of xenobiotics metabolism over the last decade. This includes detection of novel cytochromes P450 and conjugating enzymes, elucidation of the chromosomal organisation of these genes, linking evolutionary relationships and classification of these enzymes by sequence alignment, identification of regulatory elements for controlled gene expression, and last but not least heterologous expression of these genes. In the mid-1980s the first successful heterologous expression of a cloned mammalian cytochrome P450 gene in yeast cells was reported (Oeda et al. 1985). Since then, this area has seen a tremendous expansion in terms of numbers of cytochromes P450 and conjugating enzymes expressed, and expression systems employed (Fig. 1).
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© 1995 Springer-Verlag Berlin Heidelberg
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Schmalix, W.A., Doehmer, J. (1995). Use of Recombinant Cells in Metabolic and Mechanistic Studies. In: Degen, G.H., Seiler, J.P., Bentley, P. (eds) Toxicology in Transition. Archives of Toxicology, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79451-3_2
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DOI: https://doi.org/10.1007/978-3-642-79451-3_2
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