Abstract
The use of autologous cultured epidermal cell sheets has become a standard method for the coverage of extensively burned patients. The disadvantages of these sheet grafts are: (a) the relatively long time needed to grow the grafts, (b) technical difficulties in applying the fragile grafts resulting in an uncertain take, and (c) high costs. In a preliminary study we applied cultured keratinocytes suspended in fibrin glue onto eight burned areas in three patients with deep second and third degree burns. Glycerol-preserved, allogenic split-thickness skin was overgrafted onto this suspension four times. Reepithelialization was very rapid and ranged from 70% to 100% for overgrafted and 30% to 100% for bare keratinocyte grafts.
Histology revealed a layered squamous epithelium and indications of partial integration of allogenic dermis. Besides the good skin quality, the new technique has the advantage that it can be easily repeated and that actively proliferating epidermal cells can be transferred.
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© 1995 Springer-Verlag Berlin Heidelberg
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Stark, G.B., Kaiser, H.W., Kopp, J., Horch, R. (1995). Cultured Autologous Keratinocytes Suspended in Fibrin Glue to Cover Burn Wounds. In: Schlag, G., Holle, J. (eds) Fibrin Sealing in Surgical and Nonsurgical Fields. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79346-2_22
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DOI: https://doi.org/10.1007/978-3-642-79346-2_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58550-3
Online ISBN: 978-3-642-79346-2
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