Abstract
Na,K-ATPase and gastric parietal cell H,K-ATPase are two P-type ATPases that have been extensively studied. P-type ATPases, dimers with distinct α and β subunits, are inhibited by orthovanadate. During the past several years isoforms of both the α and β subunits of Na, K-ATPase and H,K-ATPase have been isolated and sequenced and, at least for the a subunit of Na,K-ATPase, there have been extensive structure-function relationship studies (Lingrel et al, 1990; Horisberger et al, 1991). Na, K-ATPase is essential for both epithelial and non-epithelial cell function by maintaining a low intracellular [Na] and a high intracellular [K] as a result of extrusion of Na in exchange for K. In most epithelial cells Na, K-ATPase is located on the basolateral membrane and until recently ouabain had been considered a specific inhibitor. Na, K-ATPase is not inhibited by either omeprazole or SCH 28080, inhibitors of gastric acid secretion and gastric H,K-ATPase which, in contrast, is located on the apical membrane of parietal cells. Gastric H,K-ATPase functions as a K- H exchange and is inhibited by omeprazole and SCH 28080, but not by ouabain (Wallmark et al, 1987). Despite these pharmacological differences, comparison of its amino acid sequence to that of the Na pump reveals partial (60%) homology (Shull amp; Lingrel, 1986).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abrahamse SL, Bindels RJM, vanOs CH (1992) The colon carcinoma cell line Caco-2 contains an H+/K+-ATPase that contributes to intracellular pH regulation. Pflugers Archiv 421: 591–597
Binder HJ, Rajendran VM (1991) Aldosterone alters active Na, CI and K transport in rat proximal and distal colon. In: Aldosterone: Fundamental Aspects. Ed. JP Bonvalet, et al Colloque INSERM John Libbey Eurotext Ltd., Paris pp 229–238
Crowson MS, Shull G (1992) Isolation and characterization of a cDNA encoding the putative distal colon H+, K+-ATPase. J Biol Chem 267: 13740–13748
del Castillo JR, Rajendran VM, Binder HJ (1991) K+-activated ATPase activities in rat distal colon: Apical membrane localization of ouabain-sensitive and ouabain-insensitive components. Am J Physiol 261: G1005–G1011
Foster ES, Hayslett JP, Binder HJ (1984) Mechanism of active potassium absorption and secretin in the rat colon. Am J Physiol 246: G611–G617
Halm DR, Frizzell RA (1986) Active K transport across rabbit distal colon: relation to Na absorption and CI secretion. Am J Physiol 251: C252–C267
Horisberger J, Lemas D, Kraehenbuhl V, Rossier BC (1991) Structure-function of Na,K-ATPase. Ann Rev Physiol 53: 565–584
Jaisser F, Coutry N, Farman N, Binder HJ, Rossier BC (1993) A putative H,K-ATPase is selectively expressed in surface epithelial cells of rat distal colon. Am J Physiol (in press)
Kaunitz JD, Sachs G (1986) Identification of a vanadate sensitive potassium-dependent proton pump from rabbit colon. J Biol Chem 261: 14005–14010
Lingrel JB, Orlowski J, Shull MM, Price EM (1990) Molecular genetics of Na, K-ATPase. Progress in Nucleic Acid Research 38: 37–89
McCabe RD, Smith PL, Sullivan LP (1984) Potassium transport by rabbit descending colon. Am J Physiol 251: C252–C267
Pandiyan V, Rajendran VM, Binder HJ (1992) Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium- depleted rat distal colon Gastroenterology 102: 1846 - 1853
Perrone RD, McBride DE (1988) Aldosterone and Pco2 enhance rubidium absorption in rat distal colon. Am J Physiol 254: G898–G906
Shull GE, Lingrel JB (1986) Molecular cloning of the rat stomach (H++ K+)-ATPase. J Biol Chem 261: 16788–16791
Suzuki Y, Kaneko K (1987) Acid secretion in isolated guinea pig colon. Am J Physiol 253: G155–G164
Suzuki Y, Kaneko K (1989) Ouabain-sensitive H+-K+ exchange mechanism in the apical membrane of guinea pig colon. Am J Physiol 256: G979–G988
Sweiry JH, Binder HJ (1989) Characterization of aldosterone- induced potassium secretion in rat distal colon. J Clin Invest 83: 844–851
Sweiry JH, Binder HJ (1990) Active potassium absorption in rat distal colon. J Physiol (London) 423: 155–170
Wallmark B, Briving C, Fryklund J, Munson K, Jackson R, Mendelein J, Rabon E, Sachs G (1987) Inhibition of gastric H+-K+- ATPase and acid secretion by SCH 28080, a substituted pyridyl (1,2a)-imidazole. J Biol Chem 262: 2077–2084
Wills NK, Biagi B (1982) Active potassium transport by rabbit descending colon epithelium. J Membr Biol 64: 195–203
Wingo CS (1989) Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct. J Clin Invest 84: 361–365
Zhou X, Wingo CS (1992) H-K-ATPase enhancement of Rb efflux by cortical collecting duct. Am J Physiol 263: F43–F48
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Binder, H.J. (1994). Apical K+-ATPase and Active Potassium Absorption in the Distal Colon. In: Hirst, B.H. (eds) Molecular and Cellular Mechanisms of H+ Transport. NATO ASI Series, vol 89. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79301-1_19
Download citation
DOI: https://doi.org/10.1007/978-3-642-79301-1_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79303-5
Online ISBN: 978-3-642-79301-1
eBook Packages: Springer Book Archive