Regulation and Properties of the Coated Vesicle Proton Pump

  • Yu Feng
  • Melanie Myers
  • Michael Forgac
Part of the NATO ASI Series book series (NATO ASI, volume 89)


Vacuolar acidification plays a crucial role in a number of basic cellular processes in eukaryotic cells (for review see Forgac, 1989). Thus, following receptor-mediated endocytosis, exposure of ligand-receptor complexes to a low pH within the endosomal compartment activates ligand-receptor dissociation, thus allowing receptor-recycling to occur. Receptor-recycling in turn controls the rate of uptake into cells of macromolecules such as LDL, transferrin and asialoglycoprotein. Receptor-recycling is also crucial in controlling the sensitivity of cells to hormones and growth factors, such as insulin and EGF. Exposure to a low pH within endosomes is also responsible for entry of the cytotoxic portions of many envelope viruses and toxins into cells. In addition to its role in the endocytic pathway, vacuolar acidification is crucial for the correct intracellular targeting of newly synthesized lysosomal enzymes from the Golgi to lysosomes, for the processing and degradation of macromolecules in secretory and digestive organelles, and for coupled transport processes in various vacuolar compartments.


Coated Vesicle MDBK Cell Vacuolar Acidification Noncatalytic Site Chloride Channel Activity 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Yu Feng
    • 1
  • Melanie Myers
    • 1
  • Michael Forgac
    • 1
  1. 1.Department of Cellular and Molecular PhysiologyTufts University School of MedicineBostonUSA

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