Advertisement

Regulation and Properties of the Coated Vesicle Proton Pump

  • Yu Feng
  • Melanie Myers
  • Michael Forgac
Part of the NATO ASI Series book series (NATO ASI, volume 89)

Abstract

Vacuolar acidification plays a crucial role in a number of basic cellular processes in eukaryotic cells (for review see Forgac, 1989). Thus, following receptor-mediated endocytosis, exposure of ligand-receptor complexes to a low pH within the endosomal compartment activates ligand-receptor dissociation, thus allowing receptor-recycling to occur. Receptor-recycling in turn controls the rate of uptake into cells of macromolecules such as LDL, transferrin and asialoglycoprotein. Receptor-recycling is also crucial in controlling the sensitivity of cells to hormones and growth factors, such as insulin and EGF. Exposure to a low pH within endosomes is also responsible for entry of the cytotoxic portions of many envelope viruses and toxins into cells. In addition to its role in the endocytic pathway, vacuolar acidification is crucial for the correct intracellular targeting of newly synthesized lysosomal enzymes from the Golgi to lysosomes, for the processing and degradation of macromolecules in secretory and digestive organelles, and for coupled transport processes in various vacuolar compartments.

Keywords

Coated Vesicle MDBK Cell Vacuolar Acidification Noncatalytic Site Chloride Channel Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adachi, I., Arai,H., Pimental,R. and Forgac,M. (1990a) “Proteolysis and Orientation on Reconstitution of the Coated Vesicle Proton Pump” J.Biol.Chem. 265, 960–966.PubMedGoogle Scholar
  2. Adachi, I., Puopolo,K., Marquez-Sterling,N., Arai,H. and Forgac,M. (1990b) “Dissociation, Crosslinking and Glycosylation of the Coated Vesicle Proton Pump” J.Biol.Chem. 265, 967–973.PubMedGoogle Scholar
  3. Arai,H., Berne,M., Terres,G., Terres,H., Puopolo,K. and Forgac,M. (1987a) “Subunit Composition and ATP-Site Labeling of the Coated Vesicle (H+)- ATPase” Biochemistry 26, 6632–6638.CrossRefGoogle Scholar
  4. Arai,H., Berne,M. and Forgac,M. (1987b) “Inhibition of the Coated Vesicle Proton Pump and Labeling of a 17,000 Dalton Polypeptide by N,N’-Dicyclo hexylcarbodiimide” J.Biol.Chem. 262, 11006–11011.Google Scholar
  5. Arai,H., Terres,G., Pink,S. and Forgac,M. (1988) “Topography and Subunit Stoi chiometry of the Coated Vesicle Proton Pump” J.Biol.Chem. 263, 8796–8802.Google Scholar
  6. Arai,H., Pink,S. and Forgac,M. (1989) “Interaction of Anions and ATP with the Coated Vesicle Proton Pump” Biochemistry 28, 3075–3082.CrossRefGoogle Scholar
  7. Feng,Y. and Forgac,M. (1992a) “Cysteine 254 of the 73-kDa A Subunit is Responsible for Inhibition of the Coated Vesicle (H+)-ATPase Upon Modification by Sulfhydryl Reagents” J.Biol.Chem. 267, 5817–5822.Google Scholar
  8. Feng,Y. and Forgac,M. (1992b) “A Novel Mechanism for Regulation of Vacuolar Acidification” J.Biol.Chem. 267, 19769–19772.Google Scholar
  9. Forgac,M. (1989) “Structure and Function of the Vacuolar Class of ATP-Driven Proton Pumps” Physiol.Rev. 69, 765–796.Google Scholar
  10. Forgac,M. (1992) “Structure and Properties of the Coated Vesicle (H+)-ATPase” J.Bioenerg.Biomemb. 24, 341–350.CrossRefGoogle Scholar
  11. Forgac,M. (1992) “Structure, Function and Regulation of the Coated Vesicle V-ATPase” J.Exp.Biol. 172, 155–169.Google Scholar
  12. Forgac,M., Cantley,L., Wiedenmann,B., Altstiel,L. and Branton,D. (1983)Google Scholar
  13. Clathrin-Coated Vesicles Contain an ATP-Dependent Proton Pump Proc.Nat1. Acad.Sci. 80, 1300–1303.Google Scholar
  14. Marquez-Sterling,N., Herman,I.M., Pesecreta,T., Arai,H., Terres,G. and Forgac,M. (1991) “Immunolocalization of the Vacuolar-Type (H+)-ATPase from Clathrin-Coated Vesicles” Eur.J.Cell Biol. 56, 19–33.Google Scholar
  15. Mulberg,A.E., Tulk,B.M. and Forgac,M. (1991) “Modulation of Coated Vesicle Chloride Channel Activity and Acidification by Reversible Protein Kinase A-Dependent Phosphorylation” J.Biol.Chem. 266 20590–20593.Google Scholar
  16. Myers,M. and Forgac,M. (1993a) “The Coated Vesicle Vacuolar (H+)-ATPase Associates with and Is Phosphorylated by the 50 kDa Polypeptide of the Clathrin Assembly Protein AP-2” J.Biol.Chem. 268, 9184–9186.PubMedGoogle Scholar
  17. Myers,M. and Forgac,M. (1993b) “Assembly of the Peripheral Domain of the Bovine Vacuolar H+-ATPase” J.Cell.Physiol. 156, 35–42.CrossRefGoogle Scholar
  18. Pearse,B.M.F. and Robinson,M.S. (1990) Ann.Rev.Cell Biol. 6, 151–171.CrossRefGoogle Scholar
  19. Puopolo,K. and Forgac,M. (1990) “Functional Reassembly of the Coated Vesicle Proton Pump” J.Biol.Chem. 265, 14836–14841.Google Scholar
  20. Puopolo,K., Kumamoto,C., Adachi,I. and Forgac,M. (1991) “A Single Gene Encodes the Catalytic “A” Subunit of the Bovine Vacuolar H+ -ATPase” J.Biol.Chem. 266 24564–24572.PubMedGoogle Scholar
  21. Puopolo,K., Kumamoto,C., Adachi,I., Magner,R. and Forgac,M. (1992a)Google Scholar
  22. Differential Expression of the “β Subunit of the Vacuolar H+-ATPase in Bovine Tissues” J.Biol.Chem. 267, 3696–3706.Google Scholar
  23. Puopolo,K., Sczekan,M., Magner,R. and Forgac,M. (1992b) “The 40-kDa Subunit Enhances but Is Not Required for Activity of the Coated Vesicle Proton Pump” J.Biol.Chem. 267, 5171–5176.PubMedGoogle Scholar
  24. Zhang,J., Myers,M. and Forgac,M. (1992) “Characterization of the Vo Domain of the Coated Vesicle (H+)-ATPase” J.Biol.Chem. 267, 9773–9778.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Yu Feng
    • 1
  • Melanie Myers
    • 1
  • Michael Forgac
    • 1
  1. 1.Department of Cellular and Molecular PhysiologyTufts University School of MedicineBostonUSA

Personalised recommendations