The Generation of Pvt-1/Ck Chimeric Transcripts as an Assay for Chromosomal Translocations in Mouse Plasmacytomas
A region of mouse chromosome 15 termed the c-Myc/Pvt-1 mega-gene locus is frequently associated with chromosomal breakpoints in a number of B-cell tumors including the mouse plasmacytoma and Burkitt’s lymphoma (for review see Potter 1990). In mouse plasmacytomas, one of two distinct types of chromosomal translocations can be found in nearly 100% of all tumors: 1). a t(12;15) translocation involving the Ig-H locus and c-Myc or 2). a t(6;15) translocation involving the Ig-k locus and Pvt-1. While the focus of such translocation events appears to be c-Myc, it is not yet clear whether Pvt-1 plays an active role in this process.
KeywordsChromosomal Translocation Breakpoint Region Translocation Breakpoint Chromosomal Breakpoint Complex Translocation
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- Huppi K, Siwarski D, Skurla RM Jr, Goodnight J and Mushinski JF (1990b) Isolation of normal and tumor-specific Pvt-1 clones. Curr Top Micro Immuol 166: 233–241Google Scholar
- Huppi K, Siwarski D, Goodnight J, Skurla RM Jr and Mushinski JF (1992) Alternative splicing of Pvt-1 transcripts in murine B-lymphocytic neoplasms accompanies amplification and chromosomal translocation. Int J of Oncol 1: 525–532Google Scholar
- Potter M, Morrison S and Miller F (1994) Plasmacytoma induction by silicone gels. Curr Top Micro Immunol (this volume) Shapiro MA and Weigert M.A (1987) A complex translocation at the murine k light-chain locus. Mol. and Cell. Biol. 7: 4130–4133Google Scholar
- Shtivelman E and Bishop JM (1989) The PVT gene frequently amplifies with MYC in tumor cells. Mol and Cell Biol. 9: 1148–1154Google Scholar
- Shtivelman E and Bishop JM (1990) Effects of translocations on transcription from PVT. Mol and Cell Biol 10: 1835–1839Google Scholar