Sequence, Expression and Function of an mRNA Encoding a Soluble Form of the Human Interleukin-6 Receptor (sIL-6R)
Hematopoietic growth factor receptors, in general, are integral membrane proteins composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular signal-transducing domain. Soluble receptors that lack the transmembrane domain have been shown to be potent immunomodulators of their respective ligands [1–6]. Since IL-6 has been shown to be a central growth factor for myeloma cells , an sIL-6R may modulate myeloma cell growth. A novel IL-6R mRNA was isolated from human myeloma cells that exhibits a deletion of the entire transmembrane domain . The corresponding cDNA was sequenced, expressed in fibroblasts, and the functional activity of the protein product was determined using an IL-6 dependent cell line.
KeywordsMyeloma Cell Human Myeloma Cell Potent Immunomodulator Human Myeloma Cell Line Translational Reading Frame
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- 2.Weisman HF, Bartow T, Leppo MK, Marsh HC, Carson GR, Concino MF, Boyle MP, Roux KH, Weisfeldt ML, Fearon DT (1990) Soluble human complement receptor type 1: in vivo inhibitor of complement suppressing post-ischemic myocardial inflammation and necrosis. Science 249: 146 - 151.PubMedCrossRefGoogle Scholar
- 6.Fanslow WC, Clifford KN, Park LS, Rubin AS, Voice RF, Beckmann MP, Widmer MB (1991) Regulation of alloreactivity in vivo by IL-4 and the soluble IL-4 receptor. J of Immunol 147: 535–540.Google Scholar