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Sequence, Expression and Function of an mRNA Encoding a Soluble Form of the Human Interleukin-6 Receptor (sIL-6R)

  • J. A. Lust
  • D. F. Jelinek
  • K. A. Donovan
  • L. A. Frederick
  • B. K. Huntley
  • J. K. Braaten
  • N. J. Maihle
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 194)

Abstract

Hematopoietic growth factor receptors, in general, are integral membrane proteins composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular signal-transducing domain. Soluble receptors that lack the transmembrane domain have been shown to be potent immunomodulators of their respective ligands [1–6]. Since IL-6 has been shown to be a central growth factor for myeloma cells [7], an sIL-6R may modulate myeloma cell growth. A novel IL-6R mRNA was isolated from human myeloma cells that exhibits a deletion of the entire transmembrane domain [8]. The corresponding cDNA was sequenced, expressed in fibroblasts, and the functional activity of the protein product was determined using an IL-6 dependent cell line.

Keywords

Myeloma Cell Human Myeloma Cell Potent Immunomodulator Human Myeloma Cell Line Translational Reading Frame 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • J. A. Lust
    • 1
  • D. F. Jelinek
    • 1
  • K. A. Donovan
    • 1
  • L. A. Frederick
    • 1
  • B. K. Huntley
    • 1
  • J. K. Braaten
    • 1
  • N. J. Maihle
    • 1
  1. 1.Department of Laboratory MedicineMayo ClinicRochesterUSA

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