Summary
The murine B-lymphocyte cell line 38C-13 is characterized by several cell surface markers typical for an early stage of B-cell differentiation. Cells of this cell line posses cell surface membrane IgM molecules composed of µ and κ polypeptide chains. They also produce “surrogate” or “pseudo” light chains (ΨL) coded by the λ5 and VpreB genes. Variants of the 38C-13 cell line which do not synthesize κ chains can be isolated from the 38C-13 population by the use of anti-idiotype antibodies in vivo and in vitro. In some κ chain-deficient variant cell lines, cells which have regained surface IgM expression but have lost the original idiotype specificity, can be isolated. This idiotype switch is probably due to a secondary rearrangment of the κ gene. In the κ chain-deficient variant cells, the ii chains assemble with the surrogate light chains but the assembled IgM-like molecules are not expressed on the cell surface. It is suggested that surrogate light chains play an important role in the induction of κ gene rearrangement but that surface expression of µ-ΨL complexes is not required for this process.
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© 1995 Springer-Verlag Berlin Heidelberg
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Caspi, Y., Taya, M., Hollander, N., Haimovich, J. (1995). Light Chain Loss and Reexpression Leads to Idiotype Switch. Surrogate Light Chains are Probably Responsible for this Process. In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1994. Current Topics in Microbiology and Immunology, vol 194. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79275-5_22
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DOI: https://doi.org/10.1007/978-3-642-79275-5_22
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