Agrobacterium rhizogenes-Mediated Transformation: Transformed Roots to Transformed Plants

  • D. Tepfer
Part of the Springer Lab Manual book series (SLM)


The simplest transformation protocol for transformation with A. rhizogenes uses plants grown in soil, producing chimeras with transgenic root systems and normal shoots (Lambert and Tepfer 1991). In species where root regeneration is spontaneous, the root systems can produce transgenic shoots, which are recognized by their altered phenotype.


Root Culture Secondary Metabolite Production Agrobacterium Rhizogenes Transgenic Shoot Morning Glory 
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© Springer-Verlag Berlin Heidelberg 1995

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  • D. Tepfer

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