Introduction II Agrobacterium-rhizogenes,a Natural Transformation System
The protocol detailed below describes an efficient method for Agrobacterium tumefaciens-mediated transformation of Brassica napus. cv Westar based on two earlier published procedures (Radke et al. 1988; De Block et al. 1989). Hypocotyl explants are cocultivated in liquid medium with the nopaline-type Agrobacterium strain MOG301 carrying different binary vectors comprising the gus-intron reporter gene and a selectable marker. Transgenic plants are produced with kanamycin, hygromycin B, phosphinothricin, or chlorsulfuron as selective agents.
KeywordsTransgenic Plant Selectable Marker Hypocotyl Explants Transgenic Callus Wash Medium
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- Vancanneyt G, Schmidt R, O’Connor-Sanchez A, Willmitzer L, Rocha-Sosa M (1990) Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250PubMedCrossRefGoogle Scholar