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Introduction II Agrobacterium-rhizogenes,a Natural Transformation System

  • J. B. Bade
  • B. Damm
Part of the Springer Lab Manual book series (SLM)

Abstract

The protocol detailed below describes an efficient method for Agrobacterium tumefaciens-mediated transformation of Brassica napus. cv Westar based on two earlier published procedures (Radke et al. 1988; De Block et al. 1989). Hypocotyl explants are cocultivated in liquid medium with the nopaline-type Agrobacterium strain MOG301 carrying different binary vectors comprising the gus-intron reporter gene and a selectable marker. Transgenic plants are produced with kanamycin, hygromycin B, phosphinothricin, or chlorsulfuron as selective agents.

Keywords

Transgenic Plant Selectable Marker Hypocotyl Explants Transgenic Callus Wash Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • J. B. Bade
  • B. Damm

There are no affiliations available

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