Abstract
The protocol described below has been used successfully to localise RNA transcripts for a number of genes expressed in various tissues (leaf, apex, flower, stem, fruit, root) of tomato (Fleming et al. 1993), tobacco (Fleming et al. 1992) and Spirodela (Smart and Fleming 1993). It is adapted from the original method described by Cox and Goldberg (1988). To minimise RNA degradation, aqueous solutions should be autoclaved (when possible), glassware baked, and sterile plasticware used. Follow standard procedures to exclude RNase contamination.
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References
Cox KH, Goldberg RB (1988) Analysis of plant gene expression. In: Shaw CH (ed) Plant molecular biology: a practical approach. IRL, Oxford, pp 1 - 34
Fleming AJ, Mandel T, Hofmann S, Sterk P, de Vries SC, Kuhlemeier C (1992) Expression pattern of a tobacco lipid transfer protein gene within the shoot apex. Plant J 2: 855 - 862
Fleming AJ, Mandel T, Roth I, Kuhlemeier C (1993) The patterns of gene expression in the tomato shoot apical meristem. Plant Cell 5: 297 - 309
Smart CC, Fleming AJ (1993) A plant gene with homology to D-myo-inositol-3-phosphate synthase is rapidly and spatially up-regulated during an abscisic acid-induced morphogenic response in Spirodela polyrrhiza. Plant J 4: 279 - 293
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© 1995 Springer-Verlag Berlin Heidelberg
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Fleming, A.J. (1995). Localization of RNA Transcripts in Plant Tissue by In Situ Hybridisation. In: Potrykus, I., Spangenberg, G. (eds) Gene Transfer to Plants. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79247-2_27
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DOI: https://doi.org/10.1007/978-3-642-79247-2_27
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48967-9
Online ISBN: 978-3-642-79247-2
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