Stable Transformation of Wheat by Microprojectile Bombardment
The following protocol describes methods that have been used successfully in the rapid production of multiple independent lines of fertile transgenic wheat (Weeks et al. 1993). Several features of the transformation protocol have been reported and used by other laboratories (Vasil et al. 1993; Becker et al. 1994; Nehra et al. 1994). These features include the biolistics-mediated gene delivery into cell tissue derived from immature embryos and, secondly, the bombardment of calli with microprojectiles coated with DNA containing the selectable marker gene, bar (phosphinothricin acetyl transferase). Selection is carried out using the herbicides bialaphos and Basta.
KeywordsEmbryogenic Callus Immature Embryo Transgenic Wheat Microprojectile Bombardment Green Sector
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