Abstract
Microspore-derived and immature zygotic embryos (MDEs and IEs) were successfully used for stable transformation of barley (Hordeum vulgare L.) through microprojectile bombardment (Wan and Lemaux 1994). A protocol describing the transformation procedure is detailed below. Two common cultivars for barley tissue culture are used, Igri (a winter cultivar) for anther culture to produce MDEs and Golden Promise (a spring cultivar) as the source of IEs. The procedure for DNA precipitation and microprojectile bombardment is essentially as described (Daines 1990). Bombarded MDEs and IEs are cultured on selection medium repeatedly to recover stably transformed callus cultures. Plants are regenerated from the transformed callus on medium containing selective agent.
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© 1995 Springer-Verlag Berlin Heidelberg
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Wan, Y., Lemaux, P.G. (1995). Biolistic Transformation of Microspore-Derived and Immature Zygotic Embryos and Regeneration of Fertile Transgenic Barley Plants. In: Potrykus, I., Spangenberg, G. (eds) Gene Transfer to Plants. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79247-2_16
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DOI: https://doi.org/10.1007/978-3-642-79247-2_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48967-9
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