Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants

Abstract

Gene constructs may be introduced into plant cells or protoplasts by a number of methods. Since the plant cell wall presents a barrier to introduction of foreign genes, removal of cell walls to produce protoplasts increases the efficiency of DNA transfer. The protocols given below describe procedures and conditions (1) for transient gene expression and (2) for stable transformation of potato by electroporation of potato protoplasts.