Studies on Persistence and Enhancement of Targeted Gene Expression
Intravenous injection of DNA-protein complexes into rats has been shown by us previously to result in transient recombinant gene expression in liver, lasting 4–5 days. The eventual deterioration of gene expression is due in part to instability of the targeted DNA. However, we noted retention of transgene sequences in liver and persistent recombinant gene expression, lasting 2–4 months, when the animals were subjected to partial hepatectomy immediately following in vivo gene transfer. Therefore, in an attempt to determine the mechanism(s) responsible for persistent gene expression following partial hepatectomy, we characterized the molecular state of the retained, liver-associated transgenes.
KeywordsPartial Hepatectomy Tumor Vaccine Modify Tumor Cell Recombinant Gene Expression Adequate Immune Response
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