Abstract
Isolated, cultured hepatocytes would seem at first sight to be an ideal model system for the study of hepatocarcinogenesis and the testing of chemicals that may be carcinogens as the liver is the major site of activation of many potential carcinogens and, thus can act as generator of the toxic metabolite as well as the target.The great potential of this model is, however, diluted by the diversity of approaches taken by various workers in the field to the preparation and culturing of hepatocytes.There is no accepted or recommended method for preparing the cells from intact liver (although many methods have been published) and little work on the optimisation of the medium and medium additions needed for culturing of hepatocytes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Guillouzo A., Morel F.,Fardel O., and Meunier B. (1993) Use of human hepatocyte cultures for drug metabolism studies.Toxicology 82:209–219.
Gustafsson J-A, Stenberg A (1974). Irreversible androgenic programming at birth of microsomal and soluble rat liver enzymes active on androst-4-ene-3,17-dione and 5a-androstane-3α,17β-diol.J.Biol.Chem 249:711–718.
Paine A (1990).The maintenance of cytochrome P$%) in rat hepatocyte cultures: Some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P450 system.Chem-biol Interact. 74:1–31.
Rogiers V.,Vercruysse A.(1993) Rat hepatocyte cultures and co-cultures in biotransformation studies of xenobiotics. Toxicology 82:193–208.
Seglen P.O. (1976) Preparation of rat liver cells.Methods Cell Biol. 13:29–83.
Skett P. (1994) Problems in using isolated and cultured hepatocytes for xenobiotic metabolism-based toxicity testing — solutions? Toxicology In Vitro (in press).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Skett, P. (1994). The Preparation and Culturing of Rat Hepatocytes. In: Skouteris, G.G. (eds) Liver Carcinogenesis. NATO ASI Series, vol 88. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79215-1_23
Download citation
DOI: https://doi.org/10.1007/978-3-642-79215-1_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79217-5
Online ISBN: 978-3-642-79215-1
eBook Packages: Springer Book Archive