Zusammenfassung
Aufgrund ihrer Bedeutung für Gewebestrukturierung und Interaktion mit extrazellulärer Matrix (ECM) wurden die Integrinmuster normaler Human-Keratinozyten, der gut differenzierenden Zellinie HaCaT, sowie von benignen und malignen HaCaT-ras Zellklonen analysiert. Vergleichbar zu Epidermis und Transplantaten normaler Zellen (auf thymus-aplastischen Nacktmäusen) war die Lokalisation der Integrine α2βl und α3βl (Immunfluoreszenz) in Epithelien von HaCaT und benignen Zellen perizellulär und weitgehend auf die Basalzellschicht beschränkt, während α6β4 größtenteils eine polare Verteilung assoziiert mit Basalmembrankomponenten zeigte. Dagegen kam es in Transplantaten maligner Zellen zu einer drastischen Expansion aller Integrine weit in suprabasale Bereiche mit gelegentlich fokal stark erhöhter Konzentration von α6β4. Diese Veränderungen waren auch in organotypischen Kokulturen maligner Zellen mit Fibroblasten manifest und dabei strikt abhängig von vitalen Funktionen der Mesenchymzellen. Die negative Korrelation der Integrinmuster mit dem Differenzierungsgrad und die verstärkte Expression in invasiven Bereichen unter Verlust der Zellpolarität deuten auf ursächliche Zusammenhänge mit malignem Wachstumsverhalten hin.
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Breitkreutz, D., Stark, HJ., Baur, M., Fusenig, N.E. (1994). Differentielle Veränderungen epidermaler Integrinmuster in Modellepithelien transformierter benigner und maligner Keratinozyten (HACAT-RAS). In: Mahrle, G., Schulze, HJ., Krieg, T. (eds) Wundheilung — Wundverschluß. Fortschritte der operativen und onkologischen Dermatologie, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79173-4_5
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