Principles of Multidimensional NMR

  • Gerhard Wagner
Part of the NATO ASI Series book series (volume 87)


Multidimensional NMR has become a key tool in chemistry and structural biology. One of the reasons for this interest is that the method can be used to solve structures of small proteins or nucleic acids in solution. Thus, NMR has evolved from an esoteric technique to a major analytical tool. NMR experiments are built from a combination of various radio frequency pulses separated by delays, and the oscillation of the radiofrequency of the pulses may have different phases with respect to a spectrometer reference phase. This allows for an essentially unlimited number of different pulse sequences, many of which can provide interesting information about molecular structure and mobility. The advent of the operator product formalism (Sørensen et al., 1983) and a general familiarity with this formalism makes the design of new and interesting pulse sequences rather simple and has lead to an avalanche of new NMR techniques. In this article, the basic principles of 1D and nD NMR experiments are introduced on a basic and pictorial level. No explanation of the concept of coherence and net magnetization, or the operator product formalism is given since these are treated in a different chapter of this book.


Nuclear Overhauser Effect Receiver Coil Coherence Transfer Heteronuclear Multiple Quantum Coherence Diagonal Peak 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Gerhard Wagner
    • 1
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA

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