At the beginning of my research career, during fellowship years at the MGH, I demonstrated for the first time that angiotensin infusion to rats stimulated aldosterone secretion by the rat adrenal gland. While in Melbourne, Australia (Monash University), I initiated work on aspects of reproduction, together with Dr. Kevin Catt developing a sensitive assay for measuring estrogen in the circulation, a protein-binding assay, before the steroid radioimmunoassay era. This assay allowed us to determine the earliest profiles of estradiol in the circulation throughout the menstrual cycle, showing the preovulatory rise of estradiol in 1970. Subsequently, we provided the first demonstration of the blockade of estradiol midcycle peak during treatment with oral contraceptive.
References and Other Sources
- Dufau ML (1990) The luteinizing hormone/human chorionic gonadotropin receptor of testis and ovary. Purification and characterization. In: Litwack G (ed) Receptor Purification vol 1. Humana Press, pp 147-171..Google Scholar
- Tsai-Morris CH, Buczko E, Wang W, Dufau ML (1990a) Intronic nature of the rat luteinizing hormone receptor gene defines a soluble receptor subspecies with hormon binding activity. Biol Chem 265:19385–19388.Google Scholar
- Ulisse A, Fabbri A, Tinajero JC, Dufau ML (1990b) A novel mechanism of action of corticotropin releasing factor in rat leydig cells. Biol Chem 265:1964–1971.Google Scholar
- Kitamura M, Buczko E, Dufau ML (1991) Dissociation of hydroxylase and lyase activities by site-directed mutagenesis of the rat P450 17a. Mol Endocrinol ?:1371-1380..Google Scholar
- Tsai-Morris, Buczko E, Wang W, Xie XZ, Dufau ML (1991) Structural organization of the rat luteinizing hormone (LH) receptor gene. Biol Chem 266:11355–11359.Google Scholar