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Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes pp 43–56Cite as

Developmental Regulation of Co-suppression In Petunia hybrida

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 197))

Abstract

The gene silencing phenomenon to be discussed here, initially termed “co-suppression” (Napoli et al. 1990; Van Der Krol et al. 1990; Jorgensen 1990), was observed in purple-flowered petunia plants genetically modified by the introduction of DNA containing a chalcone synthase coding sequence under the control of the strong CaMV 35S promoter and the 3′ end from the nopaline synthase gene of Agrobacterium. The selectable marker gene consisting of the coding sequence for neomycin phosphotransferase under the control of nopaline synthase promoter and with the 3′ end from the octopine synthase gene was also inserted on the same T-DNA. These genes were introduced into petunia cells via the transferred portion of the Ti plasmid of Agrobacterium tumefaciens (i.e. the T-DNA).

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Author notes

  1. S. Bonhomme

    Present address: INRA Centre de Versailles, Station de Genetique et Amelioration des Plantes, 78026, Versailles Cedex, France

Authors and Affiliations

  1. John Innes Centre, Norwich Research Park, NR4 7UH, Colney, Norwich, UK

    R. B. Flavell, M. O’Dell, M. Metzlaff, S. Bonhomme & P. D. Cluster

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  1. R. B. Flavell
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  2. M. O’Dell
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  3. M. Metzlaff
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  4. S. Bonhomme
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  5. P. D. Cluster
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  1. Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, Carl-von-Linné-Weg 10, 50829, Köln, Germany

    Peter Meyer

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© 1995 Springer-Verlag Berlin Heidelberg

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Flavell, R.B., O’Dell, M., Metzlaff, M., Bonhomme, S., Cluster, P.D. (1995). Developmental Regulation of Co-suppression In Petunia hybrida . In: Meyer, P. (eds) Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes. Current Topics in Microbiology and Immunology, vol 197. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79145-1_4

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  • DOI: https://doi.org/10.1007/978-3-642-79145-1_4

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