Advertisement

Integration of Acquired Thermotolerance within the Developmental Program of Seed Reserve Mobilization

  • John J. Burke
Part of the NATO ASI Series book series (volume 86)

Abstract

Abiotic stresses alter seedling metabolism from imbibition to seedling emergence resulting in stand establishment delays, poor stand quality, and often regional catastrophic crop losses. Temperature stress is the most universal of all the stresses associated with germination and stand establishment. The present study evaluated changes in acquired thermotolerance during the developmentally programmed mobilization of seed reserves for early seedling growth in cucumber (Cucumis sativus L.). To accomplish this, the development of a new protocol for evaluating thermotolerance was required because of limitations inherent in existing viability assays. The procedure developed for this study took advantage of the temperature sensitivity of chlorophyll synthesis and enabled the detection of cellular injury at temperatures 8 to 10°C below those identified for high temperature-induced injury by the standard triphenyltetrazolium chloride reduction test. Cellular injury at temperatures similar to those reported previously for cellular regrowth studies could be seen with the Chi accumulation test. Developmental changes in inherent and acquired thermotolerance following germination were identified using this protocol. An inherent resistance observed during the first days following planting was lost with increasing seedling age, and the range of temperatures responsible for the induction of acquired thermotolerance narrowed with age. Seedlings grown at different temperatures exhibited differences in the time required for the induction of acquired thermotolerance when exposed to 40°C. In summary, this study has identified developmental changes in the inducibility and magnitude of acquired thermotolerance in cucumber cotyledons through the use of a novel viability assay.

Keywords

Germination Percentage Electrolyte Leakage Heat Shock Response Temperature Block Primed Seed 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abemethy RH, Thiel DS, Petersen NS, Helm KW (1989) Thermotolerance is developmentally dependent in germinating wheat seed. Plant Physiol 89: 569–576CrossRefGoogle Scholar
  2. Anion DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24: 1–15CrossRefGoogle Scholar
  3. Bond U, Schlesinger MJ (1987) Heat shock proteins and development. Adv Genet 24: 1–28PubMedCrossRefGoogle Scholar
  4. Burke JJ, Oliver MJ (1993) Optimal Thermal Environments for Plant Metabolic Processes (Cucumis sativus L.). Light harvesting chlorophyll a/b pigment-protein complex of photosystem II and Seedling Establishment in Cucumber. Plant Physiol 102: 295–302Google Scholar
  5. Caldwell CR (1993) Estimation and analysis of cucumber (Cucumis sativus L.) leaf cellular heat sensitivity. Plant Physiol 101: 939–945PubMedGoogle Scholar
  6. Cantiiffe DJ (1981) Priming of lettuce seed for early and uniform emergence under conditions of environmental stress. Acta Hort 22: 29–38Google Scholar
  7. Carpenter WJ (1989) Salvia splendens seed pregermination and priming for rapid and uniform plant emergence. J Amer Soc Hort Sci 114: 247–250Google Scholar
  8. Carpenter WJ, Boucher JF (1991) Priming improves high-temperature germination in pansy seed. HortSci 26: 541–544Google Scholar
  9. Helm KW, Abemethy RH (1990) Heat shock protein and their mRNAs in dry and early imbibing embryos of wheat. Plant Physiol 93: 1626–1633PubMedCrossRefGoogle Scholar
  10. Helm KW, Petersen NS, Abemethy RH (1989) Heat shock response in germinating embryos of wheat Plant Physiol 90: 598–605Google Scholar
  11. Heydecfeer W (1977) Stress and seed germination: An agronomic view, p. 237–276. In: AA Khan (ed.). The physiology and biochemistry of seed dormancy and germination. North Holland Publishing Co., Amsterdam.Google Scholar
  12. Krishnan M, Nguyen HT, Burke JJ (1989) Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol 90: 140–145PubMedCrossRefGoogle Scholar
  13. Parera CA, Cantiiffe DJ (1992) Priming leek seed for improved germination and emergence at high temperature. HortSci 27: 1077–1079Google Scholar
  14. Rumpel J, Szudyga I (1978) The influence of pre-sowing seed treatments on germinations and emergence of tomato ‘New Yorker’ at low temperatures. Sci Hort 9: 119–125CrossRefGoogle Scholar
  15. Simmonds J (1980) Increasing seedling establishment of Impatiens wallerana in response to low temperature or polyethylene glycol seed treatments. Can J Plant Sci 60: 561–569CrossRefGoogle Scholar
  16. Vierling E (1991) The roles of heat shock proteins in plants. Ann Rev Plant Physiology Plant Mol Biol 42: 579–620CrossRefGoogle Scholar
  17. Wang C, Lin B-L (1993) The disappearance of an hsc70 species in mung bean seed during germination: purification and characterization of the protein. Plant Mol Biol 21: 317–329PubMedCrossRefGoogle Scholar
  18. Wu M-T, SJ Wallner (1983) Heat stress responses in cultured plant cells. Development and comparison of viability tests. Plant Physiol 72: 817–820Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • John J. Burke
    • 1
  1. 1.Plant Stress and Water Conservation ResearchUSDA-ARSLubbockUSA

Personalised recommendations