Regulation of Low Temperature-Induced Genes during Cold Acclimation of Arabidopsis Thaliana
Arabidopsis thaliana provides an ideal model system for molecular analysis of plant cold acclimation. This small Cruciferae can readily cold acclimate and the acclimation process is accompanied by expression of a specific set of low temperature-responsive genes. Structural analysis of such Iti (low temperature induced) genes has demonstrated that many of them code for polypeptides related to members of the RAB/LEA/DHN family of water stress responsive proteins. This structural similarity is indicative of common function for these proteins and suggests overlapping responses to freezing and desiccation stress. The other Iti genes characterized seem to code for novel, often very hydrophilic proteins. Enhanced freezing tolerance of A. thaliana can be induced by exposure to low temperature, mild desiccation or exogenous abscisic acid (ABA). Mutant studies have suggested that ABA-controlled processes appear to be required for a normal acclimation response. In accordance with the observed pattern of freezing tolerance induction, expression of the lti genes is responsive to the same three stimuli. However, there are marked differences in the expression patterns of the lti genes during the different modes of induction. Furthermore, the genes seem to fall into three different categories with respect to the signal pathways employed for their expression. Existence of separate response pathways to the different stimuli appears to be the most common mechanism for induction of these genes. Accordingly, stimulus specific DNA elements appear to be present in the lti promoters.
KeywordsSerine Polypeptide Dehydration Alanine Boiling
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- Koornneef M, Jorna ML, Brinkhorst-van der Swan DLC, Karssen CM (1982) The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana ( L.) Heynh. Theor Appl Genet 61: 385–393Google Scholar
- Kurkela S, Franck M, Heino P, Lamp;ng V, Palva ET (1988) Cold induced gene expression in Arabidopsis thaliana L. Plant Cell Reports 7: 495 - 498Google Scholar
- Kurkela S (1991) Structure and expression analysis of an Arabidopsis thaliana gene family of two cold induced genes. PhD Thesis, University of Helsinki, Helsinki, FinlandGoogle Scholar
- Levitt J (1980) Responses of plants to environmental stresses: Vol 1. Chilling, freezing and high temperature stresses. Academic Press, New YorkGoogle Scholar
- Ling V, Heino P, Palva ET (1989) Low temperature acclimation and treatment with exogenous abscisic acid induce common polypeptides in Arabidopsis thaliana ( L.) Heynh. Theor Appl Genet 77: 729–734Google Scholar
- Meyerowitz EM (1989) Arabidopsis, a useful weed. Cell 56: 263–269Google Scholar
- Nordin K, Lang V, Mantyla E, Heino P, Welin B, Baudo M, Holmstrom K-O, Palva ET (1993a) Role of ABA in regulation of low temperature-induced genes in Arabidopsis thaliana. In Advances in Plant Cold Hardiness, edited by P.H. Li and L. Christersson, pp. 45–56. CRC Press, Boca Raton, FloridaGoogle Scholar
- Nordin K, Vahala T, Palva ET (1993b) Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana ( L.) Heynh. Plant Mol Biol 21: 641–653Google Scholar
- Orr W, Keller WA, Singh J (1986) Induction of freezing tolerance in an embryogenic cell suspension culture of Brassica napus by abscisic acid at room temperature. J Plant Physiol 126: 23–32Google Scholar
- Sakai A, Larcher W (1987) Frost survival of plants. Responses and adaptations to freezing stress. Springer-Verlag, BerlinGoogle Scholar
- Stebbins GL (1972) Flowering plants: Evolution above the species level. Belknap, Harvard BostonGoogle Scholar