Advertisement

Developmental regulation and enhancement of heat shock gene expression

  • F. Schöffl
  • E. Kloske
  • A. Wagner
  • K. Severin
  • G. Schröder
Conference paper
Part of the NATO ASI Series book series (volume 86)

Abstract

The heat shock (hs) response is one of the best-characterized gene regulatory systems in plants that is controlled by environmental stress. It affects a number of genes encoding hs proteins (HSPs), which become transcriptionally activated by the interaction between cis-regulatory promoter elements (HSE) and the trans-active hs activator protein HSF. In current research the molecular mechanism of transcriptional regulation is much emphasized. Soybean hs genes have played a key role in the identification of cis-active hs promoter elements. Their faithful regulation and use in chimeric constructions in transgenic tobacco and Arabidopsis suggested that a highly conserved and general regulatory mechanism of the hs response exists in plants. The isolation and characterization of genes encoding HSF of tomato confirmed this model by demonstrating the binding of HSF to synthetic HSEs (Scharf et al., 1990), and by transient activation of heterologous hs promoters, including also the. soybean Gmhspl7.3-B promoter, in tobacco protoplasts (Treuter et al., 1993). The functional analysis of HSF from tomato and Arabidopsis and the manipulation of HSF expression in transgenic plants will have a major impact on our understanding of the molecular basis of the signal transfer induced by environmental factors.

Keywords

Transgenic Plant Transgenic Tobacco Developmental Regulation Heat Shock Gene Heat Stress Transcription Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baumann G, Raschke E, Bevan M and Schoffl F (1987) Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants. EMBO J 6: 1161–1166PubMedGoogle Scholar
  2. Bouchard RA (1990) Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes. Genome 33: 68–79PubMedCrossRefGoogle Scholar
  3. Breyne P, Van Montagu M, Depicker A and Gheysen G (1992) Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco. Plant Cell 4: 463–471PubMedCrossRefGoogle Scholar
  4. Cockerill PN and Garrard WT (1986) Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44: 273–282PubMedCrossRefGoogle Scholar
  5. Czarnecka E, Key JL, and Gurley WB (1989) Regulatory domains of the Gmhsp 17.5-E heat shock promoter of soybean: a mutational analysis. Mol Cell Biol 9: 3457–3463PubMedGoogle Scholar
  6. Dietrich PS, Bouchard RA, Casey ES and Sinibaldi RM (1991) Isolation and characterization of a small heat shock protein gene from maize. Plant Physiol 96: 1268–1276PubMedCrossRefGoogle Scholar
  7. Gasser SM and Laemmli UK (1986) Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster. Cell 46: 521–530PubMedCrossRefGoogle Scholar
  8. Gasser SM and Laemmli UK (1987) A glimpse at chromosomal order.Trends in Genet 3: 16–22Google Scholar
  9. Gyorgyey J, Gartner A, Nemeth K, Magyar Z, Hirt H, Heberle-Bors E and Dutits D (1991) Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. PI Mol Biol 6: 999–1007CrossRefGoogle Scholar
  10. Hall JG, Allen GC, Loer DS, Thompson WF and Spiker S (1991) Nuclear scaffolds and scaffold-attachment regions in higher plants. Proc Natl Acad Sci USA 88: 9320–9324PubMedCrossRefGoogle Scholar
  11. Helm KW and Abernethy RH (1990) Heat shock proteins and their mRNAs in dry and early imbibing embryos of wheat. Plant Physiol 93: 1626–1633PubMedCrossRefGoogle Scholar
  12. Hernandez LD and Vierling E (1993) Expression of low molecular weight heat shock proteins under field conditions. Plant Phys 101: 1209–1216Google Scholar
  13. Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A and Hoffmann N (1984) Inheritance of functional foreign genes in plants. Science 223: 496–498PubMedCrossRefGoogle Scholar
  14. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG and Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231CrossRefGoogle Scholar
  15. Howarth C (1989) Heat shock proteins in Sorghum bicolor and Pennisetum americanum. 1. Genotypic and developmental variation during seed germination. Plant Cell Environ 12: 471–477CrossRefGoogle Scholar
  16. Jackson DA (1986) Organization beyond the gene. Trends Biol Sci 6: 249–252 Jacobs M, Dolferus R and Van Den Bossche D (1988) Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants of Arabidopsis thaliana ( L.) Heynh. Biochem Genet 26: 105–122CrossRefGoogle Scholar
  17. Joergenson R (1990) Altered gene expression in plants due to trans interactions between homologous genes. TIBTECH 8: 340–344Google Scholar
  18. Kloske E et al. (1993) Developmental regulation and tissue specific differences in the expression of chimeric hs-GUS genes in transgenic tobacco and Arabidopsis plants, (submitted)Google Scholar
  19. Krol AR van der, Mur LA, Beld M, Mol JNM and Stuitje AR (1990) Flavonoid genes in Petunia: Addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2: 291–299PubMedCrossRefGoogle Scholar
  20. Laemmli UK, Kas E, Poljak L and Adachi Y (1992) Scaffold-associated regions: cis-acting determinants of chromatin structural loops and functional domains. Curr Opin Gent Dev 2: 275–285CrossRefGoogle Scholar
  21. Marrs KA, Casey ES, Capitant SA, Bouchard RA, Dietrich PS, Mettler IJ, and Sinibaldi R (1993) Characterization of two maize HSP90 heat shock protein genes and expression during heat shock, embryogenesis, and pollen development. Dev Genetics, 14: 27–41CrossRefGoogle Scholar
  22. Matzke MA and Matzke AJM (1990) Gene interactions and epigenetic variation in transgenic plants. Dev. Genet. 11: 214–223CrossRefGoogle Scholar
  23. Matzke MA, Priming M, Trnovsky J and Matzke AJM (1989) Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants. EMBO J 8: 643–649PubMedGoogle Scholar
  24. Meyer P, Heidmann I and Niedenhof I (1993) Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia. Plant J 4: 89–100PubMedCrossRefGoogle Scholar
  25. Mielke C, Kohwi Y, Kohwi-Shigematsu T, Bode J (1990) Hierarchical binding of DNA fragments derived from scaffold-attachment regions: Correlation of properties in vitro and function in vivo. Biochem 29: 7475–7485CrossRefGoogle Scholar
  26. Napoli C, Lemieux C and Joergensen R (1990) Introduction of a chimeric chalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2: 279–289PubMedCrossRefGoogle Scholar
  27. Rieping M and Schoffl F (1992) Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimeric heat shock genes in transgenic tobacco. Mol Gen Genet 321: 226–232Google Scholar
  28. Scharf K-D, Rose S, Zott W, Schoffl F and Nover L (1990) Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF. EMBO J, 9: 4495–4501PubMedGoogle Scholar
  29. Schoffl F, Rieping M, Baumann G, Bevan M and Angermiiller S (1989) The function of plant heat shock promoter elements in the regulated expression of chimeric genes in transgenic tobacco. Mol Gen Genet 217: 246–253PubMedCrossRefGoogle Scholar
  30. Schoffl F, Diedring V, Kliem M, Rieping M, Schroder G and Severin K (1992) The heat shock response in transgenic plants: the use of chimeric heat shock genes. In Inducible plant proteins. Their biochemistry and molecular biology, ( Wray JL, ed). Cambridge: Cambridge University Press, pp 267–288Google Scholar
  31. Schoffl F, Schroder G, Kliem M and Rieping M (1993) A SAR sequence containing 395 bp fragment mediates enhanced,,gene dosage-correlated expression of a chimeric heat shock gene in transgenic tobacco plants. Transgenic Research 2: 20–27Google Scholar
  32. Severin K, Wagner A and Schoffl F (1993) Heat-inducible Adh transgenes in Arabidopsis: reporter of developmental control of the heat shock response and selectable marker for the isolation of mutants, (submitted)Google Scholar
  33. Slatter RE, Dupree P and Gray JC (1991) A scaffold-associated DNA region is located downstream of the pea plastocyanin gene. Plant Cell 3: 1239–1250PubMedCrossRefGoogle Scholar
  34. Treuter E, Nover L, Ohme K and Scharf K-D (1993) Promoter specificity and deletion analysis of three tomato heat stress transcription factors. Mol Gen Genet (in press)Google Scholar
  35. van Herpen MMA, Reijnen WH, Schrauwen JAM, de Groot PF, Jager JWH and Wullems GJ (1989) Heat shock proteins and survival of germinating pollen of Lilium longiflorum and Nicotiana tabacum. J Plant Physiol 134: 345–351Google Scholar
  36. Vierling E. and Sun A (1987) Developmental expression of heat shock proteins in higher plants. In: Environmental Stress in Plants ( Cherry J, ed). Berlin: Springer, pp 343–354Google Scholar
  37. Xiao CM and Mascarenhas JP (1985) High-temperature induced thermotolerance in pollen tubes of Tradescantia and heat-shock proteins. Plant Physiol 78: 887–890PubMedCrossRefGoogle Scholar
  38. Zimmerman JL, Apuya N, Darwish K and O’Carrol C (1989) Novel regulation of heat shock genes in carrot somatic embryo development. Plant Cell 1: 1137–1146PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • F. Schöffl
    • 1
  • E. Kloske
    • 1
  • A. Wagner
    • 1
  • K. Severin
    • 1
  • G. Schröder
    • 1
  1. 1.Lehrstuhl für Allgemeine GenetikUniversität TübingenTübingenGermany

Personalised recommendations