Developmental regulation and enhancement of heat shock gene expression

  • F. Schöffl
  • E. Kloske
  • A. Wagner
  • K. Severin
  • G. Schröder
Conference paper
Part of the NATO ASI Series book series (volume 86)


The heat shock (hs) response is one of the best-characterized gene regulatory systems in plants that is controlled by environmental stress. It affects a number of genes encoding hs proteins (HSPs), which become transcriptionally activated by the interaction between cis-regulatory promoter elements (HSE) and the trans-active hs activator protein HSF. In current research the molecular mechanism of transcriptional regulation is much emphasized. Soybean hs genes have played a key role in the identification of cis-active hs promoter elements. Their faithful regulation and use in chimeric constructions in transgenic tobacco and Arabidopsis suggested that a highly conserved and general regulatory mechanism of the hs response exists in plants. The isolation and characterization of genes encoding HSF of tomato confirmed this model by demonstrating the binding of HSF to synthetic HSEs (Scharf et al., 1990), and by transient activation of heterologous hs promoters, including also the. soybean Gmhspl7.3-B promoter, in tobacco protoplasts (Treuter et al., 1993). The functional analysis of HSF from tomato and Arabidopsis and the manipulation of HSF expression in transgenic plants will have a major impact on our understanding of the molecular basis of the signal transfer induced by environmental factors.


Transgenic Plant Transgenic Tobacco Developmental Regulation Heat Shock Gene Heat Stress Transcription Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • F. Schöffl
    • 1
  • E. Kloske
    • 1
  • A. Wagner
    • 1
  • K. Severin
    • 1
  • G. Schröder
    • 1
  1. 1.Lehrstuhl für Allgemeine GenetikUniversität TübingenTübingenGermany

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