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Electrophoresis of Enzymes pp 141–179Cite as

Chemistry of Enzyme Visualization

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Abstract

The basic principle of enzyme visualization in situ is to present an enzyme with a solution containing an enzyme specific substrate. Demonstration of an enzyme is achieved if the catalytic action of the enzyme on this substrate produces a coloured reaction product. Often, however, the primary reaction products are colourless and require coupling with a visualizing agent to generate a coloured, preferably insoluble, final reaction product.

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  1. Institut für Allgemeine Botanik, Fachbereich Biologie, Johannes Gutenberg-Universität, D-55099, Mainz, Germany

    Professor Dr. Gunter M. Rothe

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  1. Professor Dr. Gunter M. Rothe
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© 1994 Springer-Verlag Berlin Heidelberg

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Rothe, G.M. (1994). Chemistry of Enzyme Visualization. In: Electrophoresis of Enzymes. Springer Labmanual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79069-0_5

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  • DOI: https://doi.org/10.1007/978-3-642-79069-0_5

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