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Electrophoresis of Enzymes pp 127–139Cite as

Sodium Dodecylsulphate Electrophoresis

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Abstract

Sodium dodecylsulphate (SDS) consists of an aliphatic chain of 12 C atoms to which at one end a sulphate residue is bound. It forms complexes with both the polar and non- polar amino acid residues of proteins irrespective of their sizes and shapes, leaving the primary structure uninfluenced. In electrophoresis SDS is used:

  1. a)

    to separate (enzyme) proteins into their monomeric constituents,

  2. b)

    to estimate the molecular mass of unfolded (and reduced) polypeptides, and

  3. c)

    to keep membrane proteins in a solubilized state.

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, Fachbereich Biologie, Johannes Gutenberg-Universität, D-55099, Mainz, Germany

    Professor Dr. Gunter M. Rothe

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  1. Professor Dr. Gunter M. Rothe
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© 1994 Springer-Verlag Berlin Heidelberg

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Rothe, G.M. (1994). Sodium Dodecylsulphate Electrophoresis. In: Electrophoresis of Enzymes. Springer Labmanual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79069-0_4

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  • DOI: https://doi.org/10.1007/978-3-642-79069-0_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79071-3

  • Online ISBN: 978-3-642-79069-0

  • eBook Packages: Springer Book Archive

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