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Extraction of Enzymes from Tissues, Cells and Cell-Organelles

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Book cover Electrophoresis of Enzymes

Part of the book series: Springer Labmanual ((SLM))

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Abstract

Microorganisms, such as bacteria, algae, moulds and others, are ruptured by sonication, by passage through a French press [1, 2] (Fig. 2.1) or a Manton-Gaulin homogenizer [3], by blending with glass beads [4], or by digesting the cell walls enzymically [5]. Extract preparation is preferably performed in the cold (+4 °C).

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  1. Institut für Allgemeine Botanik, Fachbereich Biologie, Johannes Gutenberg-Universität, D-55099, Mainz, Germany

    Professor Dr. Gunter M. Rothe

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Rothe, G.M. (1994). Extraction of Enzymes from Tissues, Cells and Cell-Organelles. In: Electrophoresis of Enzymes. Springer Labmanual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79069-0_2

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