Carbon- and Hydrogen-Isotope Discrimination in Crassulacean Acid Metabolism

  • H. Ziegler
Part of the Ecological Studies book series (ECOLSTUD, volume 114)


Plants have different means of adapting their metabolic, especially their photosynthetic, activity to short- or long-lasting water stress. The CO2-concentrat- ing mechanism of C4 photosynthesis, for example, allows carbon assimilation at low water cost. CAM in xerophytes - at least in plants with water-storage tissues - is an important adaptation in environments with intermittent water supply. The most effective protection against the detrimental effects of drought is the ability of plants (“resurrection plants”, “poikilohydric plants”) or parts of plants (spores, pollens, seeds, or fruits) to dry out without permanent injury and to restore their full metabolic capacity after rehydration. Interestingly, poikilohydric higher plants never show C4 or CAM photosynthesis (Table 22.1).


Crassulacean Acid Metabolism Tissue Water Resurrection Plant Crassulacean Acid Metabolism Plant Deuterium Content 
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  1. Bokhoven C, Theeuwen HHJ (1956) Deuterium content of some natural organic substances. Ned Akad Wet B 59: 78–83Google Scholar
  2. Craig H (1961) Standard for reporting concentrations of deuterium and oxygen-18 in natural waters. Science 133: 1833–1834PubMedCrossRefGoogle Scholar
  3. Ehleringer JR, Schulze ED, Ziegler H, Lange OL, Farquhar GD, Cowan IR (1985) Xylem-tapping mistletoes: water or nutrient parasites? Science 227: 1479–1481PubMedCrossRefGoogle Scholar
  4. Estep MF, Hoering TC (1981) Stable hydrogen isotope fractionation during autotrophic and mixotrophic growth of microalgae. Plant Physiol 67: 474–477PubMedCrossRefGoogle Scholar
  5. Kraus RG (1993) Ökophysiologie chilenischer Kakteen. Doctoral Thesis, Technische Universität, MünchenGoogle Scholar
  6. Lüttge U, Stichler W, Ziegler H (1985) Isotope ratios (δ 13C and δD) of nectar in comparison to tissues in C3 and CAM plants. Isr J Bot 34: 103–112Google Scholar
  7. Marshall JD, Ehleringer JR (1990) Are xylem tapping mistletoes partially heterotrophic? Oecologia 84: 244–248Google Scholar
  8. Medina E, Lüttge U, Leal F, Ziegler H (1991) Carbon and hydrogen isotope ratios in bromeliads growing under different light environments in natural conditions. Bot Acta 104: 47–52Google Scholar
  9. Osmond CB (1975) Environmental control of photosynthetic options in crassulacean acid metabolism plants. In: Marcelle R (ed) Environmental and biological control of photosynthesis. Junk, The Hague, pp 299–309Google Scholar
  10. Osmond CB, Winter K, Ziegler H (1982) Functional significance of different pathways of CO2 fixation in photosynthesis. In: Lange OL, Noble PS, Osmond CB, Ziegler H (eds) Physiological plant ecology II. Encyclopedia of plant physiology, new series, vol 12 B. Springer, Berlin Heidelberg New York, pp 479–547CrossRefGoogle Scholar
  11. Schiegl WE (1970) Natural deuterium in biogenic materials. Influence of environment and geophysical applications. PhD Thesis, University of South Africa, PretoriaGoogle Scholar
  12. Schiegl WE (1972) Deuterium content of peat as a paleoclimatic recorder. Science 175: 512–513PubMedCrossRefGoogle Scholar
  13. Schulze ED, Lange OL, Ziegler H, Gebauer G (1991) Carbon and nitrogen isotope ratios of mistletoes growing on nitrogen and non-nitrogen fixing hosts and on CAM plants in the Namib desert confirm partial heterotrophy. Oecologia 88: 457–462CrossRefGoogle Scholar
  14. Smith BN, Epstein S (1970) Biogeochemistry of the stable isotopes of hydrogen and carbon in salt marsh biota. Plant Physiol 46: 738–742PubMedCrossRefGoogle Scholar
  15. Smith BN, Ziegler H (1990) Isotopic fractionation of hydrogen in plants. Bot Acta 103: 335–342Google Scholar
  16. Smith BN, Ziegler H, Lipp J (1991) Isotopic evidence for mesophyll reduction in Zea mays, a NADP-malic enzyme plant. Naturwissenschaften 78: 358–359CrossRefGoogle Scholar
  17. Sternberg LO, DeNiro MJ, Johnson HB (1984) Isotope ratios of cellulose from plants having different photosynthetic pathways. Plant Physiol 74: 557–561PubMedCrossRefGoogle Scholar
  18. Szarek SR, Johnson HB, Ting IP (1973) Drought adaption in Opuntia basilaris. Significance of recycling carbon through crassulacean acid metabolism. Plant Physiol 52: 539–541PubMedCrossRefGoogle Scholar
  19. Urey HC, Brickwedde FG, Murphy GM (1932) A hydrogen isotope of mass 2 and its concentration. Phys Rev 39: 164CrossRefGoogle Scholar
  20. Washburn EW, Smith ER (1934a) An examination of water from various sources for variation in isotopic composition. J Res Nat Bur Stand 12: 305–311Google Scholar
  21. Washburn EW, Smith ER (1934b) The isotopic fractionation of water by physiological processes. Science 79: 188–189PubMedCrossRefGoogle Scholar
  22. Widmann K (1991) Vergleichende Untersuchungen zum Stickstoff- und Wasserhaushalt sukkulenter Pflanzen. Doctoral Thesis, Technische Universität MünchenGoogle Scholar
  23. Winter K (1979) δ 13C values of some succulent plants from Madagascar. Oecologia 40: 103–112CrossRefGoogle Scholar
  24. Ziegler H (1979) Diskriminierung von Kohlenstoff- und Wasserstoffisotopen: Zusammenhänge mit dem Photosynthesemechanismus und den Standortbedingungen. Ber Dtsch Bot Ges 92: 169–184Google Scholar
  25. Ziegler H (1986) Control of photosynthesis by variation of diffusion resistance in mistletoes and their hosts. In: Marcelle R, Clijstra H, von Poucke M (eds) Biological control of photosynthesis. Martinus Nijhoff, Dordrecht, pp 171–185CrossRefGoogle Scholar
  26. Ziegler H (1989) Hydrogen isotope fractionation in plant tissues. In: Rundel PW, Ehleringer JR, Nagy KA (eds) Stable isotopes in ecological research. Springer, Berlin Heidelberg New York, pp 105–123CrossRefGoogle Scholar
  27. Ziegler H (1994) Deuterium content in organic material of hosts and their parasites. In: Schulze E-D, Caldwell M (eds) Ecophysiology of photosynthesis. Springer, Berlin Heidelberg New York, pp 393–408Google Scholar
  28. Ziegler H, Osmond CB, Stichler W, Trimborn P (1976) Hydrogen isotope discrimination in higher plants: correlation with photosynthetic pathway and environment. Planta 128: 85–92CrossRefGoogle Scholar
  29. Ziegler H, Stichler W, Maurizio A, Vorwohl G (1977) Die Verwendung stabiler Isotope zur Charakterisierung von Honigen, ihrer Herkunft und ihrer Verfälschung. Apidologie 8: 337–347CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • H. Ziegler
    • 1
  1. 1.Institut für Botanik und MikrobiologieTechnische Universität MünchenMünchenGermany

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