Direct Somatic Embryogenesis

  • John J. Finer
Part of the Springer Lab Manual book series (SLM)

Abstract

Direct somatic embryogenesis is the formation of somatic embryos or embryo-genic tissue directly from the explant without the formation of an intermediate callus phase (Raghavan 1986). In embryogenesis systems, this is almost always what happens (Merkle et al. 1990; Finer 1994). The production of a callus usually is undesirable if embryogenesis is the goal of the research. Two known exceptions to direct embryogenesis are carrot (see Chap. 7) and alfalfa, where a mass of cells that is relatively unorganized can form embryos after the proper manipulations. Carrot and alfalfa have become regular and useful systems for embryogenesis studies due to the relative ease of producing plants in vitro. Unfortunately, in most other plants somatic embryogenesis is more difficult to obtain, but good progress is being made (Attree and Fowke 1993).

Keywords

Burner Surfactant Sucrose Maize Agar 

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References and Advanced Readings

  1. Armstrong CL, Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214CrossRefGoogle Scholar
  2. Attree SM, Fowke LC (1993) Embryogeny of gymnosperms: advances in synthetic seed technology of conifers. Plant Cell Tissue Organ Cult 35: 1–35CrossRefGoogle Scholar
  3. Finer JJ (1988) Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 7: 238–241CrossRefGoogle Scholar
  4. Finer JJ (1994) Plant regeneration via embryogenic suspension cultures. In: Dixon RA, Gonzales RA (eds) Plant cell culture: a practical approach. Oxford University Press, Oxford, pp 67–102Google Scholar
  5. Lazzeri PA, Hildebrand DF, Collins GB (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol Biol Rep 3: 160–167CrossRefGoogle Scholar
  6. Merkle SA, Parrott WA, Williams EG (1990) Applications of somatic embryogenesis and embryo cloning. In: Bhojwani SS (ed) Plant tissue culture: applications and limitations. Elsevier, Amsterdam, pp 67–102CrossRefGoogle Scholar
  7. Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5: 285–297CrossRefGoogle Scholar
  8. Raghavan V (1986) Embryogenesis in angiosperms: a developmental and experimental study. Cambridge University Press, New YorkGoogle Scholar
  9. Vain P, Yean H, Flament P (1989) Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3. Plant Cell Tissue Organ Cult 18: 143–151CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • John J. Finer

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