Sterile Techniques

  • Oluf L. Gamborg
  • Gregory C. Phillips
Part of the Springer Lab Manual book series (SLM)


The applications of plant cell, tissue and organ culture require sterile techniques. The maintenance of aseptic or sterile conditions is essential for successful tissue culture procedures. The importance of keeping the air, surfaces and floors free of dust and grime cannot be overestimated (see Chap. 1). All operations are carried out in Laminar Flow Sterile Cabinets or equivalent. The cabinets in use for most plant work have a horizontal airflow from back towards the front. Biocontainment or vertical airflow hoods may be preferable when working with pathogens or genetically engineered organisms using recombinant DNA.


Demineralized Water Fume Hood Sterile Technique Laminar Flow Hood Commercial Bleach 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Oluf L. Gamborg
  • Gregory C. Phillips

There are no affiliations available

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