The applications of plant cell, tissue and organ culture require sterile techniques. The maintenance of aseptic or sterile conditions is essential for successful tissue culture procedures. The importance of keeping the air, surfaces and floors free of dust and grime cannot be overestimated (see Chap. 1). All operations are carried out in Laminar Flow Sterile Cabinets or equivalent. The cabinets in use for most plant work have a horizontal airflow from back towards the front. Biocontainment or vertical airflow hoods may be preferable when working with pathogens or genetically engineered organisms using recombinant DNA.
KeywordsDemineralized Water Fume Hood Sterile Technique Laminar Flow Hood Commercial Bleach
Unable to display preview. Download preview PDF.
- Bragt JV, Mossel DAA, Pierik RLM, Veldstra H (eds) (1971) Effects of sterilization on components in nutrient media. H Veenman & Zonen NV, Wageningen, PO Box 117, 6700 WageningenGoogle Scholar
- Chandler SF, Thorpe TA (1985) Culture of plant cells: techniques and growth media. In: Techniques in life sciences. Cl. Setting up and maintenance of tissue and cell cultures. Elsevier Scientific, New York, pp 1–21Google Scholar
- PhytaSource (1994) Technical Bulletin. Sigma Chemical Company, St Louis, MO 63178Google Scholar
- Tisserat B, Jones D, Galletta PD (1992) Microwave sterilization of plant tissue culture media.HortScience 27: 358–361Google Scholar