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Sterile Techniques

  • Oluf L. Gamborg
  • Gregory C. Phillips
Part of the Springer Lab Manual book series (SLM)

Abstract

The applications of plant cell, tissue and organ culture require sterile techniques. The maintenance of aseptic or sterile conditions is essential for successful tissue culture procedures. The importance of keeping the air, surfaces and floors free of dust and grime cannot be overestimated (see Chap. 1). All operations are carried out in Laminar Flow Sterile Cabinets or equivalent. The cabinets in use for most plant work have a horizontal airflow from back towards the front. Biocontainment or vertical airflow hoods may be preferable when working with pathogens or genetically engineered organisms using recombinant DNA.

Keywords

Demineralized Water Fume Hood Sterile Technique Laminar Flow Hood Commercial Bleach 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bragt JV, Mossel DAA, Pierik RLM, Veldstra H (eds) (1971) Effects of sterilization on components in nutrient media. H Veenman & Zonen NV, Wageningen, PO Box 117, 6700 WageningenGoogle Scholar
  2. Buter B, Pescitelli SM, Berger K, Schmid JE, Stamp P (1994) Autoclaved and filter sterilized liquid media in maize anther culture: significance of activated charcoal. Plant Cell Rep 13: 79–82CrossRefGoogle Scholar
  3. Chandler SF, Thorpe TA (1985) Culture of plant cells: techniques and growth media. In: Techniques in life sciences. Cl. Setting up and maintenance of tissue and cell cultures. Elsevier Scientific, New York, pp 1–21Google Scholar
  4. Gamborg OL, Davis BP, Stahlhut RW (1983) Somatic embryogenesis in cell cultures of Glycine species. Plant Cell Rep 2: 209–212CrossRefGoogle Scholar
  5. Hamill SD, Sharrock SL, Smith MK (1993) Comparison of decontamination methods used in initiation of banana tissue cultures from field-collected suckers. Plant Cell Tissue Organ Cult 33: 343–346CrossRefGoogle Scholar
  6. PhytaSource (1994) Technical Bulletin. Sigma Chemical Company, St Louis, MO 63178Google Scholar
  7. Schenk N, Hsiao K-C, Bornman CH (1991) Avoidance of precipitation and carbohydrate breakdown in autoclaved plant tissue culture media. Plant Cell Rep 10: 115–119CrossRefGoogle Scholar
  8. Tisserat B, Jones D, Galletta PD (1992) Microwave sterilization of plant tissue culture media.HortScience 27: 358–361Google Scholar
  9. White DWR, Voisey C (1994) Prolific direct plant regeneration from cotyledons of white clover. Plant Cell Rep 13: 303–308CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Oluf L. Gamborg
  • Gregory C. Phillips

There are no affiliations available

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