Abstract
Microprojectile bombardment into plant cells was first described by Sanford et al. in 1987. Tungsten microprojectiles of approximately 4μm in size were accelerated into onion epidermal cells and directly visualized using an inverted microscope. Transient gene expression in onion and later in maize (Klein et al. 1988) proved in concept that tungsten microprojectiles could carry functional DNA into intact plant cells. The β-glucuronidase gene (GUS, Jefferson et al. 1987) or an anthocyanin gene from maize (Ludwig et al. 1990) has furthered the development of improved procedures with biolistic technology because transformed cells can be directly visualized.
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© 1995 Springer-Verlag Berlin Heidelberg
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Tomes, D.T., Ross, M.C., Songstad, D.D. (1995). Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment. In: Gamborg, O.L., Phillips, G.C. (eds) Plant Cell, Tissue and Organ Culture. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79048-5_16
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DOI: https://doi.org/10.1007/978-3-642-79048-5_16
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