Protoplast Isolation and Culture
Protoplasts can be isolated from plant tissues or cultured cells by enzymatic digestion to remove the cell walls. The enzymes for this purpose are preparations which are commercially available. The success of protoplast isolation depends especially on the condition of the tissue and the combination of enzymes being used. There is no standard method for the isolation and culture of protoplasts. However, the procedure follows a general pattern. The individual cell or tissue source may require special conditions for successful isolation or for culture. Protoplasts from cell suspension cultures generally are the most readily obtained and usually regenerate into dividing cells at a reasonable frequency. Moreover, if plant regeneration is possible from the cells of the suspension culture, the same is often true for the cells regenerated from the protoplasts derived from the culture. Leaf mesophyll cells from a wide range of plants also have been used as protoplast sources with success.
KeywordsSucrose Filtration Agar EDTA Steam
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References and Advanced Readings
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