Time-Lapse Video Light Microscopic and Electron Microscopic Observations of Vertebrate Epithelial Cells Exposed to Crocidolite Asbestos
The most popular hypothesis for how asbestos transforms cells is based on its physical dimensions, not its chemistry. This view is derived from the finding that the cytotoxic effects and transforming ability of various mineral fibers are related primarily to fiber dimensions (i.e., the length/width aspect ratio — See Hesterberg and Barrett, 1984; Stanton et al 1981; reviewed in Harington, 1981). For this reason, a knowledge of how mineral fibers behave within interphase and dividing cells, and with what intracellular components they interact, becomes important in elucidating the mechanism(s) by which they cause cell injury.
KeywordsAcridine Orange Asbestos Fiber Mineral Fiber Cytoplasmic Microtubule Surrounding Membrane
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- Alberts B, Bray D, Lewis J, Raff M, Roberts K, and Watson JD (1989) Molecular Biology of the Cell. Garland Publ, New York, 2nd Ed pp 323–340Google Scholar
- Barrett JC (1991) Role of chromosomal mutations in asbestos-induced cell transformation. In CC Harris, JF Lechner and BR Brinkley (eds) Cellular and Molecular Aspects of Fiber Carcinogenesis. Cold Spring Harbor Laboratory Press, pp 27–39Google Scholar
- Broaddus VC, Yung R, Sanan D, Sheppard D, Boylan AM (1993) Attachment of crocidolite asbestos fibers to pleural mesothelial cells via integrin αVß5. Mol Boil Cell Vol 4, Suppl p 284aGoogle Scholar
- Davis JMG (1967) The effects of chrysotile asbestos dust on lung macrophages maintained in organ culture: an electron-microscope study. Brit J Exp Pathol 48: 379–385Google Scholar
- Holtzman E (1989) Lysosomes. Plenum Press New York LondonGoogle Scholar
- Johnson NF and Davies R (1980) The effect of crocidolite and chrysotile on peritoneal macrophages: a study by transmission and scanning electron microscopy. In RC Brown, M Chamberlain, R Davies and IP Gormley (eds) The in vitro, effects of mineral dusts. Academic Press New York pp 97–103Google Scholar
- Lechner JF, Gerwin BI, Reddel RR, Gabrielson EW, Van der Meeren A, Linnainmaa K, Somers ANA and Harris CC (1991) Studies on human mesothelial cells: effects of growth factors and asbesti-form fibers. In CC Harris, JF Lechner, and BR Brinkley (eds) Cellular and Molecular Aspects of Fiber Carcinogenesis. Cold Spring Harbor Laboratory Press pp 115–129Google Scholar
- Ostergren G (1945) Transverse equilibria on the spindle. Bot Not 4: 467–468Google Scholar
- Rieder CL and Bowser SS (1987) Correlative light and electron microscopy on the same epoxy section. In MA Hyatt (ed) Correlative Microscopy in Biology: Instrumentation and Methods. Academic Press New York pp 249–277Google Scholar
- Rieder CL, Sluder G and Brinkley, BR (1991) Some possible routes for asbestosinduced aneuploidy during mitosis in vertebrate cells. In CC Harris, JF Lechner and BR Brinkley (eds) Cellular and Molecular Aspects of Fiber Carcinogenesis. Cold Spring Harbor Laboratory Press pp 1–26Google Scholar
- Schroer TA and Sheetz MP (1991) Functions of microtube-based motors. Ann Rev Physiol 53:629–652Google Scholar