Abstract
Although the introduction of viral inactivation methods has dramatically reduced the capacity of clotting factors to transmit viral infections, rare transmissions continue to occur. The polymerase chain reaction (PGR) is an extremely sensitive technique that can detect minute amounts of viral genomic material in these products. It has been used to show contamination of factor VIII concentrates with hepatitis C (HCV), human immunodeficiency virus (HIV), hepatitis A (HAV) and parvovirus B19. In the case of HCV and HAV it has been shown that detection of viral product in the concentrate is associated with transmission of infection to the recipient.
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Makris, M. (1994). Use of the Polymerase Chain Reaction to Detect Viral Contamination of Clotting Factor Concentrates. In: Scharrer, I., Schramm, W. (eds) 24. Hämophilie-Symposion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79009-6_12
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DOI: https://doi.org/10.1007/978-3-642-79009-6_12
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