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Measurement of passive and active force in single isolated smooth muscle cells

  • R van Mastrigt
  • J J Glerum
  • R Schot
Conference paper
Part of the NATO ASI Series book series (volume 84)

Abstract

In urodynamics, a subspecialism of urology, the function of the urinary tract is studied from a physical point of view, and methods are developed to objectively (and eventually automatically) diagnose the condition of the urinary tract. An important aspect of the function of the lower urinary tract is the contractility of the urinary bladder. Methods to measure this contractility are based on models developed using mechanical measurements on samples of bladder tissue. Such methods do not lead to a complete understanding of contractile properties of the (smooth muscle) tissue as the organization and structure of this tissue are irregular. Therefore measurements on single smooth muscle cells are necessary. Such measurements would also enable the use of smooth muscle biopsies for diagnostic reasons. In this chapter the development and first results of a practical method for measuring active and passive mechanical forces in single smooth muscle cells of the urinary bladder are described. Basically, such a method requires that viable and contractile cells can be isolated from the surrounding tissue and be attached to a transducer with sufficient resolution and stability to measure the very small forces involved. The three main issues isolation, attachment and force measurement will be separately discussed, and some first results will be shown.

Keywords

Smooth Muscle Cell Urinary Bladder Active Force Cell Length Bladder Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Douglas WR (1972). Of pigs and men and research. Space Life Sci 3: 226–234.ADSCrossRefGoogle Scholar
  2. van Dijk AM, Wieringa PA, van der Meer M, Laird JD (1984). Mechanics of resting isolated single vascular smooth muscle cells from bovine coronary artery. Am J.Physiol. 246: C277–C287.Google Scholar
  3. Fay FS (1977). Isometric contractile properties of single isolated smooth muscle cells. Nature 265: 553–556.ADSCrossRefGoogle Scholar
  4. Glerum JJ, van Mastrigt T, Romijn JC, Griffiths DJ (1987). Isolation and individual electrical stimulation of single smooth-muscle cells from the urinary bladder of the pig. J.M.Res.Cell Mot. 8: 125–134.CrossRefGoogle Scholar
  5. Glerum JJ, van Mastrigt R (1990a). Mechanical properties of mammalian single smooth muscle cells. I. A low cost large range microforce transducer. J.M.Res.Cell Mot. 11: 331–337.CrossRefGoogle Scholar
  6. Glerum JJ, van Mastrigt R (1990b). Mechanical properties of mammalian single smooth muscle cells. II. Evaluation of a modified technique for attachment of cells to the measurement apparatus. J.M.Res.Cell Mot. 11: 338–343.CrossRefGoogle Scholar
  7. Glerum JJ, van Mastrigt R (1990c). Mechanical properties of mammalian single smooth muscle cells. III. Passive properties of pig detrusor and human a terme uterus cells. J.M.Res.Cell Mot. 11: 453–462.CrossRefGoogle Scholar
  8. Glerum JJ (1991). Mechanical properties of mammalian single smooth muscle cells. PhD Thesis Erasmus University Rotterdam.Google Scholar
  9. Gosling JA, Dixon JS, Humpherson JR (1983). Gross and microscopic anatomy of the urinary bladder. In: Functional anatomy of the urinary tract. Gower Medical Publishing, London.Google Scholar
  10. Schot R, van Asselt E, van Mastrigt R (1993). A method for isolating smooth muscle cells from pig urinary bladder with low concentrations of collagenase and papain: The relation between calcium concentration and isolated cell length. Urol.Res. 21: 49–53.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • R van Mastrigt
    • 1
  • J J Glerum
    • 1
  • R Schot
    • 1
  1. 1.dept.Urology-urodynamics, Room EE1630Erasmus University RotterdamRotterdamthe Netherlands

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