The Mitochondrial Outer Membrane Contains at Least Two Distinct Channels

  • M. Thieffry
  • F. Fèvre
  • M. Pelleschi
  • J. P. Henry
Part of the NATO ASI Series book series (volume 83)


The presence in the mitochondrial outer membrane of a cationic channel blocked by a 13 residue addressing peptide had been shown by the technique of “tip-dip”. This channel was studied in planar bilayers and compared to the anionic porin, the voltage-dependent anion channel, observed in the same conditions. The two activities were clearly different. The peptide sensitive channel, present in both wild type and porin-deficient mutant yeast, is not a rescue channel, but an alternate independent permeability pathway. The channel interacted more strongly with a longer extension of the addressing peptide containing 22 residues, suggesting a physiological interaction of this type of molecule with the cationic channel.


Adrenal Cortex Cationic Channel Cationic Selectivity Planar Bilayer Basic Peptide 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • M. Thieffry
    • 1
  • F. Fèvre
    • 1
  • M. Pelleschi
    • 2
  • J. P. Henry
    • 2
  1. 1.Laboratoire de Neurobiologie Cellulaire et MoléculaireCNRSGif-sur-Yvette CedexFrance
  2. 2.Service de Neurobiologie Physico-Chimique, URA-CNRS 1112Institut de Biologie Physico-ChimiqueParisFrance

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