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Designing Probe Sets for the Detection of Chromosome Abnormalities in Acute Myeloid Leukemia Using Fluorescence In Situ Hybridization

  • K. Fischer
  • C. Scholl
  • G. Cabot
  • M. Moos
  • R. Schlenk
  • P. Theobald
  • R. Haas
  • M. Bentz
  • P. Lichter
  • H. Döhner
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 37)

Abstract

In recent years, the karyotype as a prognostic factor in acute myeloid leukemia (AML) has gained considerable interest. Clonal chromosome aberrations are identified in 50–60% of patients with AML (for review see [1]). The most frequent numerical abnormalities are trisomy 8 (+8), monosomy 7 (-7) and nullisomy Y, and the most frequent structural aberrations are t(15;17), t(8;21), abnormalities of band 16q22 (abnl 16q22), deletions of the long arm of chromosomes 5 [del(5q)] and 7 [del(7q)], translocations involving band 11q23 [t(9;11) and others], del(9q), del(11q), del(12p), and del(2oq). The frequency of these chromosome aberrations varies from approximately 10% (+8) to less than 1% [del(12p)] with conventional cytogenetic analysis.

Keywords

Acute Myeloid Leukemia Yeast Artificial Chromosome Conventional Cytogenetic Analysis Yeast Artificial Chromosome Clone Clonal Aberration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bloomfield CD and de la Chapelle A: Chromosome abnormalities in acute nonlymphocytic leukemia: Clinical and biologic significance. Semin Oncol 14: 372–378, 1987.PubMedGoogle Scholar
  2. 2.
    Fourth International Workshop on Chromosomes in Leukemia, 1982: Clinical significance of chromosomal abnormalities in acute nonlymphoblastic leukemia. Cancer Genet Cytogenet 11:332–350, 1984.Google Scholar
  3. 3.
    Arthur DC, Berger R, Golomb HM, Swansbury GJ, Reeves BR, Alimena G, van den Berghe H, Bloomfield CD, de la Chapelle A, Dewald GW, Garson OM, Hagemeijer A, Kaneko Y, Mitelman F, Pierre RV, Ruutu T, Sakurai M, Lawler SD and Rowley JD: The clinical significance of karyotype in acute myelogenous leukemia. Cancer Genet Cytogenet 40: 203, 1989.PubMedCrossRefGoogle Scholar
  4. 4.
    Bloomfield CD: Prognostic factors for selecting curative therapy for adult myeloid leukemia. Leukemia 6(Suppl 4): 65–67, 1992.PubMedGoogle Scholar
  5. 5.
    Cremer T, Landegent J, Brückner A, Scholl HP, Schardin M, Hager HD, Devilee P, Pearson P, van der Ploeg M: Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: Diagnosis of trisomy 18 with probe L1.84. Hum Genet 74:346–352, 1986.PubMedCrossRefGoogle Scholar
  6. 6.
    Döhner H, Arthur DC, Ball ED, Sobol RE, Davey FR, Lawrence D, Gordon L, Patil SR, Surana RB, Testa JR, Verma RS, Schiffer CA, Wurster-Hill DH, Bloomfield CD: Trisomy 13 — A new recurring chromosome abnormality in acute leukemia. Blood 76:1614–1621, 1990.PubMedGoogle Scholar
  7. 7.
    McDermid HE, Duncan AMV, Higgins MJ, Hamerton JL, Rector E, Brasch KR, White BN: Isolation and characterization of an α-satellite repeated sequence from human chromosome 22. Chromosoma 94: 228–234, 1986.PubMedCrossRefGoogle Scholar
  8. 8.
    Lichter P and Cremer T: Chromosome analysis by non-isotopic in situ hybridization. In: Rooney DE, Czepulkowski BH (eds.). Human Cytogenetics. Oxford University Press, New York 1:157–192, 1992.Google Scholar
  9. 9.
    Stilgenbauer S, Döhner H, Bulgay-Mörschel M, Weitz S, Bentz M, Lichter P: Retinoblastoma gene deletion in chronic lymphoid leukemias: A combined metaphase and interphase cytogenetic study. Blood 81: 2118–2124, 1993.PubMedGoogle Scholar
  10. 10.
    Bentz M, Cabot G, Moos M, Speicher MR, Ganser A, Lichter P, Döhner H: Detection of chimeric bcr-abl genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. Blood April 1994, in press.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • K. Fischer
    • 1
  • C. Scholl
    • 1
  • G. Cabot
    • 1
  • M. Moos
    • 1
  • R. Schlenk
    • 1
  • P. Theobald
    • 1
  • R. Haas
    • 1
  • M. Bentz
    • 2
  • P. Lichter
    • 2
  • H. Döhner
    • 1
  1. 1.Medizinische Klinik und Poliklinik VUniversität HeidelbergHeidelbergGermany
  2. 2.Organisation Komplexer GenomeDeutsches KrebsforschungszentrumHeidelbergGermany

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