Designing Probe Sets for the Detection of Chromosome Abnormalities in Acute Myeloid Leukemia Using Fluorescence In Situ Hybridization
In recent years, the karyotype as a prognostic factor in acute myeloid leukemia (AML) has gained considerable interest. Clonal chromosome aberrations are identified in 50–60% of patients with AML (for review see ). The most frequent numerical abnormalities are trisomy 8 (+8), monosomy 7 (-7) and nullisomy Y, and the most frequent structural aberrations are t(15;17), t(8;21), abnormalities of band 16q22 (abnl 16q22), deletions of the long arm of chromosomes 5 [del(5q)] and 7 [del(7q)], translocations involving band 11q23 [t(9;11) and others], del(9q), del(11q), del(12p), and del(2oq). The frequency of these chromosome aberrations varies from approximately 10% (+8) to less than 1% [del(12p)] with conventional cytogenetic analysis.
KeywordsAcute Myeloid Leukemia Yeast Artificial Chromosome Conventional Cytogenetic Analysis Yeast Artificial Chromosome Clone Clonal Aberration
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