Combination of rhSCF + rhG-SCF, But Not rhG-CSF Alone Potentiate the Mobilization of Hematopoietic Stem Cells with Increased Repopulating Ability into Peripheral Blood of Mice

  • N. J. Drize
  • J. L. Chertkov
  • A. R. Zander
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 37)

Abstract

The early hematopoietic progenitor including cells capable for long-term maintenance of hematopoiesis circulates in low amount in peripheral blood during steady-state conditions [1–4]. However, these progenitors were found to belong to one of more mature category in the hierarchy of hematopoietic stem cells (HSC). Indeed, in competitive repopulation assays the progeny of peripheral blood HSC is rapidly replaced by the progeny of bone marrow stem cells [5,6]. During last decade it was repeatedly demonstrated that some hematopoietic cytokines affect not only survival, proliferation and differentiation of hematopoietic cells, but also capable to mobilize HSC into circulation [7–11]. Such ability of cytokines is studying very intensively both in experimental and clinical conditions because mobilized HSC can be used itself or in combination with bone marrow cells as a source of progenitors for transplantation [12]. Among cytokines capable to mobilize HSC into circulation the most attention are payed now on SCF and its combination with other growth factors, especially G-CSF [7,13,14]. Inspite of the cytokine-mobilized HSC (usually after intensive course of chemotherapy in the period of regeneration of hematopoiesis) are now used in patients, a lot of questions has no answer. Up to now it is obscure if peripheral blood progenitors are mobilized from bone marrow by pharmacological doses of cytokines or their expansion in bone marrow and/or peripheral blood take place. For G-CSF it was shown that more probable mechanism is HSC mobilization [7,15]. The data about similar effect of G-CSF plus SCF combination was not published; meanwhile, SCF in combination with other cytokines produces in vitro more strong effect on HSC proliferation than G-CSF alone [14,16]. We have no data about the optimal time of peripheral blood HSC (pbHSC) collection: is it necessary to collect pbHSC during their highest concentration or later in the cytokine course when pbHSC level is not so high, but quality of progenitors possibly better, for example because of enrichment of population by more immature members of HSC compartment? Here these questions were addressed on murine system with use of rhG-CSF and rhSCF.

Keywords

Migration Leukemia Hydrocortisone Heparin Glutamine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Metcalf D, Moore MAS: Haemopoietic cells. North Holland, Publishing Company, Amsterdam-London, 1971Google Scholar
  2. 2.
    Barnes DWH, Loutit JF: Haemopoietic stem cells in the peripheral blood. Lancet ii:1138, 1967CrossRefGoogle Scholar
  3. 3.
    Goodman JW, Hodgson GS: Evidence for stem cells in the peripheral blood. Blood 19:702, 1962PubMedGoogle Scholar
  4. 4.
    Fliedner TM, Steinbach KH: Repopulating potential of hematopoietic precursor cells. Blood Cells 14:393, 1988PubMedGoogle Scholar
  5. 5.
    Micklem HS, Anderson N, Ross E: Limited potential of circulating hemopoietic stem cells. Nature 256: 41, 1975PubMedCrossRefGoogle Scholar
  6. 6.
    Chertkov JL, Gurevitch OA, Udalov GA: Self-maintenance ability of circulating hemopoietic stem cells. Exp Hematol 18: 90, 1982Google Scholar
  7. 7.
    Gordon MY: Physiological mechanisms in BMT and haemopoiesis — revisited. Bone Marrow Transplantation 11:193, 1993PubMedGoogle Scholar
  8. 8.
    Molineux D, Migdalska A, Szmitkowski M, Zsebo K, Dexter TM: The effect on hematopoiesis of recombinant stem cell factor (ligand for c-kit) administered in vivo to mice either alone or in combination with granulocyte colony-stimulating factor. Blood 78: 961, 1991PubMedGoogle Scholar
  9. 9.
    Drize NJ, Gan OI, Zander AR: Effect of recombinant human granulocyte colony-stimulating factor treatment of mice on spleen colony-forming unit number and self-renewal capacity. Exp Hematol 21: 1269, 1993Google Scholar
  10. 10.
    Goodnough LT, Anderson KC, Kurtz S, Lane TA, Pisciotto PT, Sayers MH, Silberstein LE: Indication and guidelines for the use of hematopoietic growth factors. Transfusion 33: 944, 1993PubMedCrossRefGoogle Scholar
  11. 11.
    McNiece IK, Briddell RA, Hartley CA, Smith KA, Andrews RG: Stem cell factor enchances in vivo effects of granulocyte colony stimulating factor for stimulating mobilization of peripheral blood progenitor cells. Stem cells 11 (supl): 36, 1993PubMedCrossRefGoogle Scholar
  12. 12.
    Zander AR, Lyding J, Bielack S: Transplantation with blood stem cells. Blood Cells 17: 301, 1991PubMedGoogle Scholar
  13. 13.
    Briddell R, Hartley C, Stoney G, McNiece I: SCF synergize with G-CSF in vivo to mobilizmurine peripheral blood progenitor cells with enchanced engraftment potential. Exp Hematol 21: 1150, 1993Google Scholar
  14. 14.
    Bodine DM, Seidel NE, Zsebo KM, Orlic D: In vivo administration of stem cell factor to mice increases the absolute number of pluripotent hematopoietic stem cells. Blood 82: 445, 1993PubMedGoogle Scholar
  15. 15.
    Tavassoli M: Expansion of blood stem cell pool or mobilization of its marrow counterpart? Exp Hematol 21: 1205, 1993PubMedGoogle Scholar
  16. 16.
    Drize NJ, Chertkov JL, Zander AR: Hematopoietic stem cell mobilization into peripheral blood of mice by combination of recombinant rat stem cell factor (rhSCF) and recombinant human granulocyte colony-stimulating factor (rhG-CSF). Manuscript in preparationGoogle Scholar
  17. 17.
    Till JE, McCulloch EA: A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Rad Res 14: 213, 1961CrossRefGoogle Scholar
  18. 18.
    Chertkov JL, Drize NJ: Cells forming spleen colonies at 7 or 11 days after injection have different proliferation rates. Cell Tissue Kinet 17: 247, 1984PubMedGoogle Scholar
  19. 19.
    Dexter TM, Allen TD, Lajtha LG: Conditions controlling the proliferation of hemopoietic stem cells in vivo. J Cell Physiol 91: 335, 1977PubMedCrossRefGoogle Scholar
  20. 20.
    Deryugina EI, Drize NJ, Olovnikova NI, Sadovnikova EYu, Chertkov JL: Primitive hemopoietic stem cell: Origin during the ontogenesis, proliferative activity and proliferative potential. Ontogenes 22: 125, 1991Google Scholar
  21. 21.
    Breivik H Hematopoietic stem cell content of murine bone marrow, spleen and blood. Limiting dilution analysis of diffusion chamber culture. J Cell Physiol 78: 73, 1971PubMedCrossRefGoogle Scholar
  22. 22.
    Deryugina EI, Drize NJ, Chertkov JL: Long-term culture inititating cells do not regenerate after irradiation. Bull Exp Biol Med 7: 96, 1987Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • N. J. Drize
    • 1
  • J. L. Chertkov
    • 1
  • A. R. Zander
    • 2
  1. 1.Dept. of Hematological Oncology and Bone Marrow TransplantationHematological Research CenterMoscowRussia
  2. 2.II Medizinische Klinik, Abt. Onkologie/HämatologieUniversitat Hamburg, Universitats-Krankenhaus EppendorfHamburgGermany

Personalised recommendations