Effects of Growth Factors In Vitro on Acute Lymphoblastic Leukemia Cells

  • G. J. L. Kaspers
  • R. Pieters
  • C. H. Van Zantwijk
  • G. H. Broekema
  • K. Hählen
  • A. J. P. Veerman
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 37)

Abstract

Growth factors may enhance the cytotoxicity of anticancer agents. Little is known about this effect of growth factors on acute lymphoblastic leukemia (ALL) cells. We studied the effects of interleukin-3 (IL-3), interleukin-7 (IL-7) and B-cell growth factor (BCGF) on cell survival and drug resistance in ALL samples, using the MTT assay.

Compared to drug-free culture without growth factors, the cell survival was significantly increased by IL- 3, IL-7 and BCGF. Compared to growth factor-free drug incubations, the cytotoxicity of thioguanine was significantly enhanced by IL- 3 and IL-7, and the cytotoxicity of cytarabine was significantly enhanced by IL-7. In contrast, the cytotoxicity of prednisolone was decreased by the growth factors in most ALL samples, and overall not significantly modulated.

We conclude that the MTT assay can be used to study the effects of growth factors on cell survival and drug resistance of ALL cells. This type of information may be useful for the design of clinical trials with growth factors in ALL.

Keywords

Toxicity Lymphoma Leukemia Resis Prednisolone 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. J. L. Kaspers
    • 1
  • R. Pieters
    • 1
  • C. H. Van Zantwijk
    • 1
  • G. H. Broekema
    • 1
  • K. Hählen
    • 2
  • A. J. P. Veerman
    • 1
  1. 1.Department of PediatricsFree University HospitalAmsterdamThe Netherlands
  2. 2.Subdivision Hemato-/OncologySophia Children’s HospitalRotterdamThe Netherlands

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