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Expression of P-Glycoprotein in Children and Adults with Leukemia — Correlation with Clinical Outcome

  • Slawomir Kaczorowski
  • Maria Ochocka
  • Robert Aleksandrowicz
  • Maria Kaczorowska
  • Michal Matysiak
  • Marek Karwacki
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 37)

Abstract

The multidrug resistance (MDR) phenomenon has been shown to be associated with the expression of P-glycoprotein (P-gp), the product of mdr-1 gene. In the present study the expression of P-gp was investigated in 40 children, 21 adults’ leukaemia and 10 healthy donors’ samples. The presence of P-gp was evaluated by the immunohistochemical method (APAAP), with three different monoclonal antibodies (MAb’s) directed against separate intra-(C219, JSB-1) and extra-cellular (MRK16) epitopes of P-gp. Human leukaemic cell line (K562) and Vincristine resistant (K562VCR) subline served as negative and positive controls for C219, JSB-1 and MRK16 staining, respectively. The expression of P-gp was found in 14 out of 40 (35%) children and in 8 out of 21 (38%) adults’ leukemia cases. No expression of P-gp in 10 healthy donors’ samples was observed. In children P-gp positive staining was detected in 3/8 Acute Myeloid Leukemia (AML), 8/26 Acute Lymphoblastic Leukemia (ALL), 1/1 Chronic Myeloid Leukemia (CML) and 2/5 Non Hodgkin’s Lymphoma (NHL) cases. In adults P-gp positive staining was noticed in 7/17 AML, 1/4 ALL cases. In children and adults samples pattern of staining for P-gp was predominantly cytoplasmic, although a Golgi-associated dot like pattern of staining was also observed. Clinical follow up in children’s and adults’ leukemias reveals good response to induction chemotherapy both in P-gp positive and negative patients.

Keywords

Acute Myeloid Leukemia Acute Lymphoblastic Leukemia Chronic Myeloid Leukemia Multidrug Resistance French American British 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Slawomir Kaczorowski
    • 1
  • Maria Ochocka
    • 2
  • Robert Aleksandrowicz
    • 3
  • Maria Kaczorowska
    • 4
  • Michal Matysiak
    • 2
  • Marek Karwacki
  1. 1.Department of ImmunologyCancer Center and Institute of OncologyWarsawPoland
  2. 2.Department of Pediatric Hematology — OncologyWarsaw University Medical SchoolWarsawPoland
  3. 3.Department of Obstetrics and GynaecologyChild and Mother InstituteWarsawPoland
  4. 4.Department of Internal Medicine — Family MedicinePostgraduate Medical CenterWarsawPoland

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