Chromatin Structure, Hyperthermia and Repair of UV-Induced DNA Photolesions in Mammalian Cells
The organization of the chromatin fibre into loops or domains by attachment to a nucleoskeleton (termed nuclear matrix or scaffold) is an essential element in current models of eukaryotic replication and transcription. The nucleoskeleton is considered to be the active site of DNA replication and transcription with templates moving past nucleoskeleton bound enzyme complexes. It is easy to visualize how DNA damage can be removed by sliding of chromatin along nucleoskeleton associated repair enzymes. Perhaps nucleoskeleton-centred repair may be confined only to biological important domains comprising active genes. Results of experiments aimed to discover nucleoskeleton-centred repair point to the existence of such a mechanism for transcriptionally active DNA.
KeywordsLithium Adenosine Pyrimidine Cyclobutane Pyrimidone
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