Reliable Chromosome Studies of Human Oocytes and Spermatozoa using the Gradual Fixation-Air Drying (GF-AD) Method

  • K. Mikamo
  • Y. Kamiguchi
  • H. Tateno
  • T. Nishino

Abstract

Recently the human oocyte chromosome study has become active owing to the progress of the in vitro fertilization method (IVF) (Zenzes and Casper 1992). The majority of the oocytes contributed for this purpose are those which are left at the second meiotic metaphase after in vitro insemination.

Keywords

Citrate Assure Trypsin Tritium Alan 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • K. Mikamo
    • 1
  • Y. Kamiguchi
    • 1
  • H. Tateno
    • 1
  • T. Nishino
    • 2
  1. 1.Department of Biological SciencesJapan
  2. 2.Department of Obstetrics and GynecologyAsahikawa Medical CollegeAsahikawa 078Japan

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