The Genetics and Physiology of the Totipotent Cell

  • Fiorella Lo Schiavo
Conference paper
Part of the NATO ASI Series book series (volume 81)


Plants, as compared to animals, are characterized by a great flexibility in their differentiative programmes. Plants are capable of regenerating part of their organs; somatic cells may become embryogenic and thus reproduce all developmental stages up to adult plants.


Maize Germinate Thymidine Sorghum Vasil 


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  1. Aleith F., Richter G. (1991) Gene expression during induction of somatic embryogenesis in carrot cell suspensions.Planta 183: 17–24CrossRefGoogle Scholar
  2. Backs-Hüsemann H., Reinert J. (1970) Embryobildung durch isolierte Einzellen aus Gewebekulturen von Daucus carota. Protoplasms 70: 49–60CrossRefGoogle Scholar
  3. Borkird C., Choi J.H., Jin Z.H., Franz G., Hatzopoulos P., Chorneau R., Bonas U., Pelegri F., Sung Z.R. (1988) Developmental regulation of embryonic genes in plants. Proc. Nat.Acad.Sci. 85: 6399–6403PubMedCrossRefGoogle Scholar
  4. Breton A.M., Sung Z.R. (1982) Temperature-sensitive carrot variants impaired in somatic embryogenesis. Dev. Biol. 90: 58–86PubMedCrossRefGoogle Scholar
  5. Choi J.H., Sung Z.R. (1984) Two-dimensional gel analysis of carrot somatic embryogenesis proteins.Plant Mol.Biol.Rep. 2: 19–25CrossRefGoogle Scholar
  6. Clark J.K., Sheridan W.F. (1991) Isolation and characterization of embryo-specific mutations of maize. Plant Cell 3: 935–951.PubMedCrossRefGoogle Scholar
  7. De Jong A.J., Cordewener J., LoSchiavo F., Terzi M., Vandekerkove J., Van Kammen A., De Vries S.C. (1992) A carrot somatic embryo mutant is rescued by chitinase. Plant Cell 4: 425–433PubMedCrossRefGoogle Scholar
  8. De Vries S.C., Booij H., Janssens R., Vogel R., Saris L., LoSchiavo F., Terzi M., van Kammen A. (1988) Carrot somatic embryogenesis depends on the phytohormone-controlled expression of correctly glycosylated extracellular proteins.Genes Dev. 2: 462–476CrossRefGoogle Scholar
  9. Dudits D., Bogre L, Gyorgyev J. (1991) Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro. J. Cell Sci. 99: 475–484.Google Scholar
  10. Filippini F., Terzi M., Cozzani F., Vallone D., Lo Schiavo F. (1992) Modulation of auxin binding proteins in cell suspensions.II. Isolation and initial characterization of carrot lines impaired in somatic embryogenesis. Theor.Appl.Genet. 84: 430–434.CrossRefGoogle Scholar
  11. Fujimura T., Komamine A. (1980) Aspects of DNA, RNA and protein synthesis during somatic embryogenesis in a Daucus carota cultivar Kurodagosun cell suspension culture. Physiol.Plant. 49: 255–260.CrossRefGoogle Scholar
  12. Gee M.A., Hagen G., Guilfoyle T.J. (1991) Tissue-specific and organ-specific expression of soybean auxin responsive transcripts GH3 and SAURs. Plant Cell 3: 419–430.PubMedCrossRefGoogle Scholar
  13. Giuliano G., LoSchiavo F., Terzi M. (1984) Isolation and developmental characterization of temperature-sensitive carrot cell variants. Theor.Appl.Genet. 67: 179–183.CrossRefGoogle Scholar
  14. Haccius B., Lakshman K.C. (1969) Adventiv-Embryonen-Embryoide-Adventiv-knospen-Ein Beitrag aus klärung der Begriffe. Osterr. bot. Z. 116: 145–158.CrossRefGoogle Scholar
  15. Halperin W. (1966) Alternative morphogenetic events in cell suspensions. Am.J.Bot. 53: 443–453.CrossRefGoogle Scholar
  16. Komamine A., Matsumoto M., Tsukahara M., Fujiwara A., Kawahara R., Ita M., Smith J., Fujimura T. (1990) Mechanisms of somatic embryogenesis in cell cultures: physiology, biochemistry and molecular biology. In “Progress in Plant Cellular and Molecular Biology” ( J.J. Nijkamp, L.H. van der Plas, J. van Aartrijk eds.) Kluwer Academic Pbshrs, Dordrecht (NL) pp. 307–313CrossRefGoogle Scholar
  17. Lo Schiavo F., Pitto L., Giuliano G., Torti G., Nuti-Ronchi V., Marazziti D., Vergara R., Orselli S., Terzi M. (1989) DNA methylation of embryogenic carrot cell cultures and its variationa as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor.Appl.Genet. 77: 325–333CrossRefGoogle Scholar
  18. Lo Schiavo F., Giuliano G., De Vries S.C., Genga A., Bollini R., Pitto L., Cozzani F., Nuti-Ronchi V., Terzi M. (1990) A carrot cell variant temperature-sensitive for somatic embryogenesis reveals a defect in the glycosylation of extracellular proteins. Mol.Gen.Genet. 233: 385–393.Google Scholar
  19. Lo Schiavo F., Filippini F., Cozzani F., Vallone D., Terzi M. (1991) Modulation of auxin binding proteins in cell suspensions. I. Differential responses of carrot embryo cultures. Plant Physiol. 97: 60–64.CrossRefGoogle Scholar
  20. Mansfield S.G., Briarty L.G. (1991) Early embryogenesis in Arabidopsis thaliana. II. The developing embryo. Canad.J.Bot. 69: 461–476.CrossRefGoogle Scholar
  21. Mayer V., Torres Ruiz R.A., Berleth T., Miséra S., Jürgens G. (1991) Mutations affecting body organization in the Arabidopsis embryo. Nature 353: 402–407CrossRefGoogle Scholar
  22. Nuti-Ronchi V., Giorgetti L., Tonelli M.G., Martini G. (1992) Ploidy reduction and genome segregation in cultured carrot cell lines. II. Somatic meiosis. Plant Cell Tiss.Org.Cult. 30: 115–120CrossRefGoogle Scholar
  23. Reinert J. (1958) Morphogenese und ihre Kontrolle an Gewebekulturen aus Karotten. Naturwiss. 45: 344–345CrossRefGoogle Scholar
  24. Schnall J.A., Cooke T.J., Cress D.E. (1988) Genetic analysis of somatic embryogenesis in carrot cell cultures:initial characterization of six classes of temperature-sensitive variants. Dev.Genet. 9: 49–67.CrossRefGoogle Scholar
  25. Sheridan W.F., Clark J.K. (1987) Maize embryogeny: a promising experimental system. Trends Genet. 3: 3–6CrossRefGoogle Scholar
  26. Skoog F., Miller C.O. (1957) In: Symp.Soc.Exp.Biol. 11: 118–131PubMedGoogle Scholar
  27. Steward F.C., Mapes M.O., Hears K. (1958) Growth and organized development of cultured cells.II. Organization in cultures grown from freely suspended cells. Am.J.Bot. 45: 705–708.CrossRefGoogle Scholar
  28. Thomas T.L., Wilde H.D. (1985) Analysis of gene expression in carrot somatic embryos. In “Somatic Embryogenesis” (M.Terzi, L. Pitto, Z.R. Sung eds.) IPRA, Rome, pp. 77–85Google Scholar
  29. Thomas T.L., Wilde H.D. (1987) Analysis of carrot somatic embryo gene expression programs. In Proc. VI Int.Congr.Plant Cell Tiss.Cult. (Alan R. Liss, New York ) pp 83–93Google Scholar
  30. Vasil I.K., Vasil V. (1986) Regeneration in cereal and other grass species. In: I.K. Vasil (ed.): Cell Culture and somatic Cell Genetics of Plants. Vol.3. Plant Regeneration and genetic Variability. Academic Press, Orlando, pp. 121–150.Google Scholar
  31. Wernicke W., Brettel. (1950) Somatic embryogenesis from Sorghum bicolor leaves. Nature 287: 138–139CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Fiorella Lo Schiavo
    • 1
  1. 1.Department of BiologyUniversity of PaduaPadovaItaly

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