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Molecular Genetic Improvement of Wheat

  • Conference paper
Plant Molecular Biology

Part of the book series: NATO ASI Series ((ASIH,volume 81))

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Abstract

Cereal grains like wheat, rice and maize were most likely amongst the first plants domesticated by prehistoric man, and ever since have exerted a dominant influence on the development of the human civilization. Even today they constitute the most important group of food plants by virtue of providing more than 50% of all food consumed by man in addition to being the principal source of calories and protein in the human diet. The yield as well as the quality of cereal crops have been greatly improved during the past several decades by the traditional methods of breeding and selection. Significant further improvement of cereal crops is, however, limited by the time consuming and labor intensive nature of plant breeding methods, the restricted gene pool, and most importantly by the severe natural barriers to hybridization with even closely related species. It is in this context that the novel methods of plant cell and molecular biology, which permit the introduction of selected and defined plant as well as bacterial, viral and other genes of interest into plants, offer new opportunities for supplementing as well as complementing plant breeding and selection for the improvement of crop plants.

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References

  • Ahmed KZ, Sagi F (1993) Culture and fertile plant regeneration from regenerable embryogenic suspension cell-derived protoplasts of wheat Triticum aestivum L.). Plant Cell Rep 12: 175–179

    Article  Google Scholar 

  • Bialy H (1992) Recalcitrant cereal: Transgenic wheat finally produced. Bio/Technology 10: 630

    Google Scholar 

  • Callis J, Fromm M, Walbot V (1987) Introns increase gene expression in cultured cells. Gens Develop 1: 1183–1200

    Article  CAS  Google Scholar 

  • Chang Y, Wang W, Warfield CY, Nguyen HT, Wong JR (1991) Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.). Plant Cell Rep 9: 611–614

    Article  Google Scholar 

  • Chibbar RN, Kartha KK, Leung N, Qureshi J, Caswell K (1991) Transient expression of marker genes in immature zygotic embryos of spring wheat (Triticum aestivum) through microprojectile bombardment. Genome 34: 453–460

    Article  CAS  Google Scholar 

  • Chin JC, Scott KJ (1977) Studies on the formation of roots and shoots in wheat callus cultures. Ann Bot 41: 473–481

    CAS  Google Scholar 

  • Christensen AH, Sharrock RA, Quail PH (1992) Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol 18: 675–689

    Article  PubMed  CAS  Google Scholar 

  • Dudits D, Nemet G, Haydu Z (1975) Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures. Canad J Bot 53: 957–963

    Article  Google Scholar 

  • Fromm M, Taylor LP, Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Nat Acad Sci USA 82: 5824–5828

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Eveleigh DE (1968) Culture methods and detection of glucanases in suspension cultures of wheat and barley. Canad J Biochem 46: 417–421

    Article  CAS  Google Scholar 

  • Gosch-Wackerle G, Avivi L, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysiol 91: 267–278

    Google Scholar 

  • Hauptmann RM, Ozias-Akins P, Vasil V, Tabaeizadeh Z, Rogers SG, Horsch RB, Vasil IK, Fraley RT (1987) Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep 6: 265–270

    Article  CAS  Google Scholar 

  • Hauptmann RM, Vasil V, Ozias-Akins P, Tabaeizadeh Z, Rogers SG, Fraley RT, Horsch RB, Vasil IK (1988) Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol 86: 602–606

    Article  PubMed  CAS  Google Scholar 

  • He DG, Yang YM, Scott KJ (1992) Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog). Plant Cell Rep 11: 16–19

    Article  CAS  Google Scholar 

  • Leemans J (1993) Ti to tomato, tomato to market: a decade of plant biotechnology. Bio/Technology 11: S22–S26

    Article  Google Scholar 

  • Li Z, Xia G, Chen H (1992) Somatic embryogenesis and plant regeneration from protoplasts isolated from embryogenic cell suspensions of wheat (Triticum aestivum L.). Plant Cell Tiss Org Cult 28: 79–85

    Article  Google Scholar 

  • Lonsdale D, Onde S, Cuming A (1990) Transient expression of exogenous DNA in intact, viable wheat embryos following particle bombardment. J Exp Bot 41: 1161–1165

    Article  CAS  Google Scholar 

  • Lorz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol Gen Genet 199: 178–182

    Article  Google Scholar 

  • Maddock SE (1987) Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.). Plant Cell Rep 6: 23–26

    Article  Google Scholar 

  • Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Oard JH, Paige D, Dvorak J (1989) Chimeric gene expression using maize intron in cultured cells of bread wheat. Plant Cell Rep 8: 156–160

    Article  CAS  Google Scholar 

  • Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): evidence for somatic embryogenesis. Protoplasma 110: 95–105

    Article  Google Scholar 

  • Ozias-Akins P, Vasil IK (1983a) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44

    Article  Google Scholar 

  • Ozias-Akins P, Vasil IK (1983b) Proliferation of and plant regeneration from the epiblast of Triticum aestivum (wheat; Gramineae) embryos. Amer J Bot 70: 1092–1097

    Article  Google Scholar 

  • Perl A, Kless H, Blumenthal A, Galili G, Galun E (1992) Improvement of plant regeneration and GUS expression in cellular wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures. Mol Gen Genet 235: 279–284

    Article  PubMed  CAS  Google Scholar 

  • Qiao YM, Cattaneo M, Locatelli F, Luppoto E (1992) Plant regeneration from long term suspension culture-derived protoplasts of hexaploid wheat (Triticum aestivum L.). Plant Cell Rep 11: 262–265

    Article  CAS  Google Scholar 

  • Redway FA, Vasil V, Lu D, Vasil IK (1990a) Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Theor Appl Genet 79: 609–617

    Article  Google Scholar 

  • Redway FA, Vasil V, Vasil IK (1990b) Characterization and regeneration of wheat (Triticum aestivum L.) from embryogenic cell suspension cultures. Plant Cell Rep 8: 714–717

    Article  CAS  Google Scholar 

  • Shimada T (1978) Plant regeneration from the callus induced from wheat embryo. Jap J Genet 53: 371–374

    Article  Google Scholar 

  • Taylor MG, Vasil V, Vasil IK (1993) Enhanced GUS gene expression in cereal/grass cell suspensions and immature embryos using the maize ubiquitin-based plasmid pAHC25. Submitted

    Google Scholar 

  • Trione EJ, Johnes LE, Metzger RJ (1968) In vitro culture of somatic wheat callus tissue. Amer J Bot 55: 529–531

    Article  Google Scholar 

  • Vasil IK (1988) Progress in the regeneration and genetic manipulation of cereal crops. Bio/Technology 6: 397–402

    Article  Google Scholar 

  • Vasil IK (1991) Plant tissue culture and molecular biology as tools in understanding plant development and in plant improvement. Curr Opin Biotech 2: 158–163

    Article  CAS  Google Scholar 

  • Vasil IK, Vasil V (1992) Advances in cereal protoplast research. Physiol Plant 85: 279–283

    Article  CAS  Google Scholar 

  • Vasil V, Brown SM, Re D, Fromm ME, Vasil IK (1991) Stably transformed callus lines from microprojectile bombardment of cell suspension cultures of wheat. Bio/Technology 9: 743–747

    Article  CAS  Google Scholar 

  • Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10: 667–674

    Article  CAS  Google Scholar 

  • Vasil V, Clancy M, Ferl RJ, Vasil IK, Hannah LC (1989) Increased gene expression by the first intron of maize Shrunken-1 locus in grass species. Plant Physiol 91: 1575–1579

    Article  PubMed  CAS  Google Scholar 

  • Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technology 8: 429–434

    Article  Google Scholar 

  • Wang YC, Klein TM, Fromm ME, Cao J, Sanford JC, Wu R (1988) Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment. Plant Mol Biol 11: 433–439

    Article  CAS  Google Scholar 

  • Yamada Y (1977) Tissue culture studies in cereals. In Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture ( Reinert J, Bajaj YPS, eds), pp 144–159. Springer-Verlag, Heidelberg

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, Gainesville, FL, 32611-0692, USA

    Indra K. Vasil & Vimla Vasil

Authors
  1. Indra K. Vasil
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  2. Vimla Vasil
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Editor information

Editors and Affiliations

  1. Department of Biology, New York University, 1009 Main Building, Washington Square, 10003, New York, NY, USA

    Gloria Coruzzi

  2. CID-CSIC, Jordi Girona, 18-26, 08034, Barcelona, Spain

    Pere Puigdomènech

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© 1994 Springer-Verlag Berlin Heidelberg

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Vasil, I.K., Vasil, V. (1994). Molecular Genetic Improvement of Wheat. In: Coruzzi, G., Puigdomènech, P. (eds) Plant Molecular Biology. NATO ASI Series, vol 81. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78852-9_49

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  • DOI: https://doi.org/10.1007/978-3-642-78852-9_49

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78854-3

  • Online ISBN: 978-3-642-78852-9

  • eBook Packages: Springer Book Archive

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