Advertisement

Clusters Of Resistance Genes in Lettuce

Map-based Cloning In Non-intensively Studied Species
  • Richard Michelmore
  • Peter Anderson
  • Patricia Okubara
  • Hanneke Witsenboer
Conference paper
Part of the NATO ASI Series book series (volume 81)

Abstract

Strategies for map-based cloning of disease resistance genes have been previously reviewed (e. g. Michelmore et al., 1993). In this chapter, we will discuss the recent progress and current limitations with particular emphasis on our efforts to clone and characterize genes for resistance to downy mildew in lettuce. The approaches discussed here are applicable to cloning the determinants of any trait for which only the genetics is known and the species has not been studied intensively at the molecular level.

Keywords

Resistance Gene Downy Mildew Disease Resistance Gene Avirulence Gene Turnip Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Crute IR and Johnson AG 1976 The genetic relationship between races of Bremia lactucae and cultivars of Lactuca sativa. Ann Appl Biol 83: 125–137CrossRefGoogle Scholar
  2. Crute IR and Dunn JA 1981 An association between resistance to root aphid (Pemphigus bursarius L.) and downy mildew (Bremia lactucae) in lettuce. Euphytica 29: 483–488CrossRefGoogle Scholar
  3. Farrara B, Ilott TW and Michelmore RW 1987 Genetic analysis of factors for resistance to downy mildew (Bremia lactucae) in species of lettuce (Lactuca sativa and L. serriola). Plant Pathol 36: 499–514.CrossRefGoogle Scholar
  4. Ferrin LJ and Camrini-Otero RD 1991 Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage. Science 254: 1494–1497PubMedCrossRefGoogle Scholar
  5. Ilott TW, Hulbert SH and Michelmore RW 1989 Genetic analysis of the gene-for-gene interaction between lettuce (Lactuca sativa) and Bremia lactucae. Phytopathology 79: 888–897.CrossRefGoogle Scholar
  6. Johal G and Briggs SP 1992 Reductase activity encoded by the Hml disease resistance gene in maize. Science 258: 985–987PubMedCrossRefGoogle Scholar
  7. Judelson HS, Tyler B, and Michelmore RW 1991 Stable transformation of the potato late blight fungus, Phytophthora infestans. Molec Plant-Microbe Interact 4: 602–607.CrossRefGoogle Scholar
  8. Kesseli RV, Paran I, Ochoa O, Wang W-C, and Michelmore RW 1990 Linkage map of lettuce (Lactuca sativa) 2N = 18. In: Genetic Maps, 6th Ed. S. J. O’Brien (ed.). Cold Spring Harbor Press. 6. 229–233.Google Scholar
  9. Koob M, Burkiewitcz A, Kur J, Szybalski W 1992 RecA-AC: Single site cleavage of plasmids and chromsomes at any predetermined restriction site. Nulc Acids Res 20: 5831–5836CrossRefGoogle Scholar
  10. Legg EJ 1991 Genetic characterization of Bremia lactucae using molecular markers. M. Sc. thesis, University of California, Davis. pp 101Google Scholar
  11. Liang P and Pardee AB 1992 Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967–971PubMedCrossRefGoogle Scholar
  12. Martin G, Ganal M, and Tanksley SD 1993 Construction of a yeast artificial chromosome library of tomato and identification of clones linked to two disease resistance loci. Mol Gen Genet 233: 25–32CrossRefGoogle Scholar
  13. Michelmore RW, Hulbert, Ilott TW and Farrara B 1988 Genetics of downy mildews. In: RH Williams and DS Ingram (eds.). Genetics of Pathogenic Fungi. Ed. GS Sidhu, Advances in Plant Pathology. Vol 6, pp. 53–79.Google Scholar
  14. Michelmore RW, Marsh E, Seely S and Landry BS 1987 Transformation of lettuce mediated by Agrobacterium tumefaciens. Plant Cell Repts 6: 439–442.Google Scholar
  15. Michelmore RW, Paran I, and Kesseli RV 1991 Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci 88: 9828–9832.PubMedCrossRefGoogle Scholar
  16. Michelmore RW, Kesseli RV, Francis DM, Paran I, Fortin MG, and Yang C-H 1992 Strategies for cloning plant disease resistance genes. In: Molecular Plant Pathology - A Practical Approach. Ed. S Gurr. IRL Press. Vol 2, pp 233–288.Google Scholar
  17. Michelmore RW, Hulbert SH, Landry BS, and Leung H 1987 Towards a molecular understanding of lettuce downy mildew. In: Genetics and Plant Pathogenesis, Eds Day PR and Jellis GJ. Blackwell Scientific. p 221–231.Google Scholar
  18. Paran I., Kesseli RV, and Michelmore RW 1991 Identification of RFLP and RAPD markers linked to downy mildew resistance genes in lettuce using near-isogenic lines. Genome 34: 1021–1027.PubMedCrossRefGoogle Scholar
  19. Paran I., Kesseli RV, and Michelmore RW 1992 Recent amplification of triose phosphate isomerase related sequences in lettuce. Genome 35: 627–635.PubMedCrossRefGoogle Scholar
  20. Paran I. and Michelmore RW 1993 Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985–993.CrossRefGoogle Scholar
  21. Pryor AJ 1987 The origin and structure of fungal disease resistance genes in plants. Trend Genet 3: 157–161CrossRefGoogle Scholar
  22. Zink FW and Duffus JE 1975 Inheritance and linkage of turnip mosaic virus susceptibility and downy mildew (Bremia lactucae) resistance in lettuce. J Amer Soc Hort Sci 98: 49–51Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Richard Michelmore
    • 1
  • Peter Anderson
    • 1
  • Patricia Okubara
    • 1
  • Hanneke Witsenboer
    • 1
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA

Personalised recommendations