Clusters Of Resistance Genes in Lettuce
Strategies for map-based cloning of disease resistance genes have been previously reviewed (e. g. Michelmore et al., 1993). In this chapter, we will discuss the recent progress and current limitations with particular emphasis on our efforts to clone and characterize genes for resistance to downy mildew in lettuce. The approaches discussed here are applicable to cloning the determinants of any trait for which only the genetics is known and the species has not been studied intensively at the molecular level.
KeywordsCorn Electrophoresis Agrobacterium Isomerase Phytophthora
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- Kesseli RV, Paran I, Ochoa O, Wang W-C, and Michelmore RW 1990 Linkage map of lettuce (Lactuca sativa) 2N = 18. In: Genetic Maps, 6th Ed. S. J. O’Brien (ed.). Cold Spring Harbor Press. 6. 229–233.Google Scholar
- Legg EJ 1991 Genetic characterization of Bremia lactucae using molecular markers. M. Sc. thesis, University of California, Davis. pp 101Google Scholar
- Michelmore RW, Hulbert, Ilott TW and Farrara B 1988 Genetics of downy mildews. In: RH Williams and DS Ingram (eds.). Genetics of Pathogenic Fungi. Ed. GS Sidhu, Advances in Plant Pathology. Vol 6, pp. 53–79.Google Scholar
- Michelmore RW, Marsh E, Seely S and Landry BS 1987 Transformation of lettuce mediated by Agrobacterium tumefaciens. Plant Cell Repts 6: 439–442.Google Scholar
- Michelmore RW, Kesseli RV, Francis DM, Paran I, Fortin MG, and Yang C-H 1992 Strategies for cloning plant disease resistance genes. In: Molecular Plant Pathology - A Practical Approach. Ed. S Gurr. IRL Press. Vol 2, pp 233–288.Google Scholar
- Michelmore RW, Hulbert SH, Landry BS, and Leung H 1987 Towards a molecular understanding of lettuce downy mildew. In: Genetics and Plant Pathogenesis, Eds Day PR and Jellis GJ. Blackwell Scientific. p 221–231.Google Scholar
- Zink FW and Duffus JE 1975 Inheritance and linkage of turnip mosaic virus susceptibility and downy mildew (Bremia lactucae) resistance in lettuce. J Amer Soc Hort Sci 98: 49–51Google Scholar