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Characterization of Protein Factors Interacting with the rDNA Promoter Region

  • Manuel Echeverria
  • Dominique Tremousaygue
  • Thomas Roscoe
  • Katia Wassmann
  • Paul Penon
  • Michel Delseny
Conference paper
Part of the NATO ASI Series book series (volume 81)

Abstract

The nuclear genes coding for ribosomal RNA are transcribed by the RNA-polymerase I system. This transcriptional machinery is, by far, much less documented than the RNA-polymerase II system which is used for protein coding genes. Before the various components of the RNA -polymerase I transcription system could be analyzed, it was necessary to elucidate the fine structure of ribosomal RNA genes in higher plants and to map the site(s) for initiation of transcription. This has been an important challenge from 1985 up to now. At the moment, rDNA spacer sequence is available for more than 15 higher plant species and the major site of transcription initiation has been determined for more than half of them. The next step in the analysis of RNA-polymerase I transcription is to identify the regulatory cis -acting sequences as well as the corresponding trans — activating factors. There are three classical and complementary methods to reach this goal.

Keywords

Initiation Site Transcription System rDNA Transcription Space Repeat Upstream Binding Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Manuel Echeverria
    • 1
  • Dominique Tremousaygue
    • 1
  • Thomas Roscoe
    • 1
  • Katia Wassmann
    • 1
  • Paul Penon
    • 1
  • Michel Delseny
    • 1
  1. 1.University of PerpignanPlant Physiology and Molecular Biology LabPerpignanFrance

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