Characterization of Protein Factors Interacting with the rDNA Promoter Region
The nuclear genes coding for ribosomal RNA are transcribed by the RNA-polymerase I system. This transcriptional machinery is, by far, much less documented than the RNA-polymerase II system which is used for protein coding genes. Before the various components of the RNA -polymerase I transcription system could be analyzed, it was necessary to elucidate the fine structure of ribosomal RNA genes in higher plants and to map the site(s) for initiation of transcription. This has been an important challenge from 1985 up to now. At the moment, rDNA spacer sequence is available for more than 15 higher plant species and the major site of transcription initiation has been determined for more than half of them. The next step in the analysis of RNA-polymerase I transcription is to identify the regulatory cis -acting sequences as well as the corresponding trans — activating factors. There are three classical and complementary methods to reach this goal.
KeywordsInitiation Site Transcription System rDNA Transcription Space Repeat Upstream Binding Factor
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