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Random Priming and Multiplex PCR with Three Short Tandem Repeats in Forensic Caseworks

  • P. Huber
  • J. Holtz
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

In our work we present two new tools for forensic caseworks. Firstly a multiplex PCR with three short tandem repeats (STRs): an easy and rapid method for individual identification with high probability. After optimization of the PCR conditions (especially temperature profile and primer concentrations) slippage bands are avoided and thereby the STRs are adapted to forensic demands. The PCR products of the chosen loci (characteristics see table 1) are simultanously detected on a single PAA-gel.

Keywords

Androgen Receptor Slippage Band Random Priming Short Tandem Repeat Androgen Receptor Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • P. Huber
    • 1
  • J. Holtz
    • 1
  1. 1.Inst. Forensic MedicineUniversity of BonnGermany

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