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Paternity Testing in Italy Using Minisatellite Variant Repeat (MVR)

  • Giuseppe Novelli
  • Stefania Lo Cicero
  • Isabella Torrente
  • Pietro Fucci
  • Bruno Dallapiccola
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

MVR-PCR provides an innovative and highly discriminating technique to assess individual variation in human DNA (1). Using MVR specific amplimers to amplify the D1S8 (MS32) locus, a ladder of PCR products corresponding to different classes of repeat units, a-type and t-type are generated to produce a diploid digital code derived from the superimposed maps of specific alleles (1). MVR-PCR can be used in pedigrees examination, forensic analysis and population studies (1,3,4).

Keywords

Repeat Unit Parentage Testing Peripheral Lymphocyte Specific Allele Type Repeat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Jeffreys A.J., Macleod A., Tamaki K., Neil D., Monckton D.G. (1991). Minisatellite repeat coding as a digital approach to DNA typing. Nature, 354, 204–209.PubMedCrossRefGoogle Scholar
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    Dover G.A. (1992). A digital DNA dipstick for probing human diversity. TIG, 8, 4547.Google Scholar
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    Monckton D.G., Tamaki K., Macleod A., Neil D.L., Jeffreys A.J. (1993). Allele-specific MVR-PCR analysis at minisatellite D1 S8. Hum.Molec.Genet., 2, 513–519.PubMedCrossRefGoogle Scholar
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    Novelli G., Lo Cicero S., Dallapiccola B. (1993). Variazioni intralleliche dei minisatelliti nell’analisi della variabilita’ genetica: MVR-PCR. In Analisi del DNA 1993. Gasparini P. Zelante L. (Edts) Incontripolymed. Firenze, 26.2. 1993, pp 19–21.Google Scholar
  5. 5.
    Novelli G. Spinelle A., Gennarelli M., Mingarelli R. and Dallapiccola B. (1992). Analysis of Apo B, HLA-DQα, and D1 S80 Polymorphisms in the Italian Population Using the Polymerase Chain Reaction. Am. J. of Hum. Biol. 4: 381–386.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Giuseppe Novelli
    • 1
  • Stefania Lo Cicero
    • 2
  • Isabella Torrente
    • 2
  • Pietro Fucci
    • 2
  • Bruno Dallapiccola
    • 2
    • 3
  1. 1.Catholic University of RomeItaly
  2. 2.Dpt. Public Health and Cell BiologyTor Vergata University of RomeItaly
  3. 3.CSS Hospital S. Giovanni RotondoItaly

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