Application of a Y-linked tetranucleotide repeat polymorphism to paternity cases

  • E. Trabetti
  • A. Casartelli
  • P. F. Pignatti
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

The polymorphic microsatellite Y-27H39 was analyzed in a large Italian population sample by the polymerase chain reaction amplification technique and a simple method of native polyacrylamide gel electrophoresis followed by ethidium bromide fluorescence. Five alleles were distinguished in 202 males, with frequencies ranging from 1% to 51%. In 52 father-son pairs, selected among 94 disputed paternity cases, previously tested with different autosomal VNTR and microsatellite regions, the Y-27H39 polymorphism confirmed all the 27 attributions and 19 of the 25 exclusions, indicating 76% informativity in Y chromosome exclusion testing.

Keywords

Bromide Electrophoresis Polyacrylamide Ethidium Ceses 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • E. Trabetti
    • 1
  • A. Casartelli
    • 1
  • P. F. Pignatti
    • 1
  1. 1.Institute of Biological SciencesUniversity of VeronaItaly

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