Application of a Y-linked tetranucleotide repeat polymorphism to paternity cases
Part of the
Advances in Forensic Haemogenetics
book series (HAEMOGENETICS, volume 5)
The polymorphic microsatellite Y-27H39 was analyzed in a large Italian population sample by the polymerase chain reaction amplification technique and a simple method of native polyacrylamide gel electrophoresis followed by ethidium bromide fluorescence. Five alleles were distinguished in 202 males, with frequencies ranging from 1% to 51%. In 52 father-son pairs, selected among 94 disputed paternity cases, previously tested with different autosomal VNTR and microsatellite regions, the Y-27H39 polymorphism confirmed all the 27 attributions and 19 of the 25 exclusions, indicating 76% informativity in Y chromosome exclusion testing.
KeywordsPaternity Testing Microsatellite Region Italian National Research Council Tandem Repeat Locus Paternity Case
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